An image analysis method for regionally defined cellular phenotyping of the
Drosophila
imaging
injury response
intestinal stem cells
midgut
organ-wide
Journal
Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676
Informations de publication
Date de publication:
27 09 2021
27 09 2021
Historique:
received:
16
02
2021
revised:
01
06
2021
accepted:
30
06
2021
entrez:
27
4
2022
pubmed:
28
4
2022
medline:
28
4
2022
Statut:
epublish
Résumé
The intestine is divided into functionally distinct regions along the anteroposterior (A/P) axis. How the regional identity influences the function of intestinal stem cells (ISCs) and their offspring remain largely unresolved. We introduce an imaging-based method, "Linear Analysis of Midgut" (LAM), which allows quantitative, regionally defined cellular phenotyping of the whole
Identifiants
pubmed: 35474669
doi: 10.1016/j.crmeth.2021.100059
pii: S2667-2375(21)00107-7
pmc: PMC9017226
doi:
Substances chimiques
Drosophila Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100059Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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