Cryo-EM Visualization of Neuronal Particles Inside Microtubules.
Cryo-EM
MAP6
Microtubule inner proteins
Microtubules
Neurons
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
27
4
2022
pubmed:
28
4
2022
medline:
30
4
2022
Statut:
ppublish
Résumé
Neuronal microtubules have long been known to contain intraluminal particles, called MIPs (microtubule inner proteins), most likely involved in the extreme stability of microtubules in neurons. This chapter describes a cryo-electron microscopy-based assay to visualize microtubules containing neuronal MIPs. We present two protocols to prepare MIPs-containing microtubules, using either in vitro microtubule polymerization assays or extraction of microtubules from mouse hippocampal neurons in culture.
Identifiants
pubmed: 35476345
doi: 10.1007/978-1-0716-1983-4_24
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
375-383Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Mitchison T, Kirschner M (1984) Dynamic instability of microtubule growth. Nature 312:237–242
doi: 10.1038/312237a0
Kapitein LC, Hoogenraad CC (2015) Building the neuronal microtubule cytoskeleton. Neuron 87:492–506
doi: 10.1016/j.neuron.2015.05.046
Baas PW, Rao AN, Matamoros AJ, Leo L (2016) Stability properties of neuronal microtubules. Cytoskeleton 73:442–460
doi: 10.1002/cm.21286
Janke C, Magiera MM (2020) The tubulin code and its role in controlling microtubule properties and functions. Nat Rev Mol Cell Biol 21:307–326
doi: 10.1038/s41580-020-0214-3
Nicastro D, Fu X, Heuser T, Tso A, Porter ME, Linck RW (2011) Cryo-electron tomography reveals conserved features of doublet microtubules in flagella. Proc Natl Acad Sci USA 108:E845–E853
doi: 10.1073/pnas.1106178108
Ichikawa M, Liu D, Kastritis PL, Basu K, Hsu TC, Yang S et al (2017) Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins. Nat Commun 8:15035
doi: 10.1038/ncomms15035
Stoddard D, Zhao Y, Bayless BA, Gui L, Louka P, Dave D et al (2018) Tetrahymena RIB72A and RIB72B are microtubule inner proteins in the ciliary doublet microtubules. Mol Biol Cell 29:2566–2577
doi: 10.1091/mbc.E18-06-0405
Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF et al (2019) Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins. Proc Natl Acad Sci U S A 116:19930–19938
doi: 10.1073/pnas.1911119116
Ma M, Stoyanova M, Rademacher G, Dutcher SK, Brown A, Zhang R (2019) Structure of the decorated ciliary doublet microtubule. Cell 179:909–922 e12
doi: 10.1016/j.cell.2019.09.030
Peters A, Proskauer CC, Kaiserman-Abramof IR (1968) The small pyramidal neuron of the rat cerebral cortex. The axon hillock and initial segment. J Cell Biol 39:604–619
doi: 10.1083/jcb.39.3.604
Rodriguez Echandia EL, Piezzi RS, Rodriguez EM (1968) Dense-core microtubules in neurons and gliocytes of the toad Bufo arenarum Hensel. Am J Anat 122:157–166
doi: 10.1002/aja.1001220110
Burton PR (1984) Luminal material in microtubules of frog olfactory axons: structure and distribution. J Cell Biol 99:520–528
doi: 10.1083/jcb.99.2.520
Garvalov BK, Zuber B, Bouchet-Marquis C, Kudryashev M, Gruska M, Beck M et al (2006) Luminal particles within cellular microtubules. J Cell Biol 174:759–765
doi: 10.1083/jcb.200606074
Atherton J, Stouffer M, Francis F, Moores CA (2018) Microtubule architecture in vitro and in cells revealed by cryo-electron tomography. Acta Cryst D Struct Biol 74:572–584
doi: 10.1107/S2059798318001948
Cuveillier C, Delaroche J, Seggio M, Gory-Faure S, Bosc C, Denarier E et al (2020) MAP6 is an intraluminal protein that induces neuronal microtubules to coil. Sci Adv 6:eaaz4344
doi: 10.1126/sciadv.aaz4344
Ramirez-Rios S, Serre L, Stoppin-Mellet V, Prezel E, Vinit A, Courriol E et al (2017) A TIRF microscopy assay to decode how tau regulates EB’s tracking at microtubule ends. Methods Cell Biol 141:179–197
doi: 10.1016/bs.mcb.2017.06.013
Bosc C, Andrieux A, Job D (2003) STOP proteins. Biochemistry 42:12125–12132
doi: 10.1021/bi0352163