Characterization and functional interrogation of the SARS-CoV-2 RNA interactome.
CP: Microbiology
SARS-CoV-2 RNA interactome
SARS-CoV-2 infection inhibitors
comprehensive identification of RNA binding proteins by mass spectrometry, ChIRP-MS
host RNA binding proteins
host-dependency factors
severe acute respiratory syndrome coronavirus 2
siRNA screen
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
26 04 2022
26 04 2022
Historique:
received:
19
03
2021
revised:
28
07
2021
accepted:
07
04
2022
entrez:
27
4
2022
pubmed:
28
4
2022
medline:
30
4
2022
Statut:
ppublish
Résumé
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the COVID-19 pandemic, which has led to a devastating global health crisis. The emergence of variants that escape neutralizing responses emphasizes the urgent need to deepen our understanding of SARS-CoV-2 biology. Using a comprehensive identification of RNA-binding proteins (RBPs) by mass spectrometry (ChIRP-MS) approach, we identify 107 high-confidence cellular factors that interact with the SARS-CoV-2 genome during infection. By systematically knocking down their expression in human lung epithelial cells, we find that the majority of the identified RBPs are SARS-CoV-2 proviral factors. In particular, we show that HNRNPA2B1, ILF3, QKI, and SFPQ interact with the SARS-CoV-2 genome and promote viral RNA amplification. Our study provides valuable resources for future investigations into the mechanisms of SARS-CoV-2 replication and the identification of host-centered antiviral therapies.
Identifiants
pubmed: 35477000
pii: S2211-1247(22)00508-3
doi: 10.1016/j.celrep.2022.110744
pmc: PMC9040432
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110744Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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