Combining in vitro and in vivo screening to identify efficient Pseudomonas biocontrol strains against the phytopathogenic bacterium Ralstonia solanacearum.
environmental microbiology
microbial ecology
microbial interactions and pathogenesis
plant-microbe interactions
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
02
02
2022
accepted:
31
03
2022
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
30
4
2022
Statut:
ppublish
Résumé
Although plant pathogens are traditionally controlled using synthetic agrochemicals, the availability of commercial bactericides is still limited. One potential control strategy could be the use of plant growth-promoting bacteria (PGPB) to suppress pathogens via resource competition or the production of antimicrobial compounds. This study aimed to conduct in vitro and in vivo screening of eight Pseudomonas strains against Ralstonia solanacearum (the causative agent of bacterial wilt) and to investigate underlying mechanisms of potential pathogen suppression. We found that inhibitory effects were Pseudomonas strain-specific, with strain CHA0 showing the highest pathogen suppression. Genomic screening identified 2,4-diacetylphloroglucinol, pyoluteorin, and orfamides A and B secondary metabolite clusters in the genomes of the most inhibitory strains, which were investigated further. Although all these compounds suppressed R. solanacearum growth, only orfamide A was produced in the growth media based on mass spectrometry. Moreover, orfamide variants extracted from Pseudomonas cultures showed high pathogen suppression. Using the "Micro-Tom" tomato cultivar, it was found that CHA0 could reduce bacterial wilt disease incidence with one of the two tested pathogen strains. Together, these findings suggest that a better understanding of Pseudomonas-Ralstonia interactions in the rhizosphere is required to successfully translate in vitro findings into agricultural applications.
Identifiants
pubmed: 35478286
doi: 10.1002/mbo3.1283
pmc: PMC9059233
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1283Informations de copyright
© 2022 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
Références
Annu Rev Phytopathol. 2003;41:117-53
pubmed: 12730389
Appl Environ Microbiol. 1997 Apr;63(4):1357-61
pubmed: 16535571
Front Microbiol. 2016 Mar 30;7:382
pubmed: 27065956
Mol Plant Pathol. 2012 Aug;13(6):614-29
pubmed: 22672649
Front Microbiol. 2017 Jun 30;8:1218
pubmed: 28713346
Int Microbiol. 2017 Dec;20(4):155-164
pubmed: 29529326
Appl Environ Microbiol. 2002 Jul;68(7):3226-37
pubmed: 12088998
AMB Express. 2019 Aug 9;9(1):125
pubmed: 31399889
Front Microbiol. 2017 Feb 03;8:100
pubmed: 28217113
Annu Rev Phytopathol. 2012;50:67-89
pubmed: 22559068
mBio. 2016 Dec 13;7(6):
pubmed: 27965449
Mol Biol Evol. 2014 Nov;31(11):2913-28
pubmed: 25086002
Appl Environ Microbiol. 1994 Jul;60(7):2553-60
pubmed: 16349332
Microb Ecol. 2009 Feb;57(2):267-75
pubmed: 19030916
Annu Rev Phytopathol. 2005;43:337-59
pubmed: 16078888
Appl Environ Microbiol. 2013 Oct;79(19):6016-22
pubmed: 23892739
Appl Environ Microbiol. 2007 Feb;73(4):1225-38
pubmed: 17189443
Front Plant Sci. 2019 Jul 19;10:845
pubmed: 31379891
Microb Ecol. 2009 Apr;57(3):501-9
pubmed: 18975025
PLoS One. 2011;6(8):e23045
pubmed: 21829688
New Phytol. 2010 Sep;187(4):920-8
pubmed: 20673287
Nat Rev Microbiol. 2005 Apr;3(4):307-19
pubmed: 15759041
Microb Ecol. 2011 May;61(4):853-9
pubmed: 21360140
Front Microbiol. 2017 Sep 26;8:1895
pubmed: 29018437
Mol Biol Evol. 2021 May 4;38(5):1792-1808
pubmed: 33306125
Molecules. 2016 Nov 09;21(11):
pubmed: 27834875
Environ Microbiol. 2021 Sep;23(9):5525-5540
pubmed: 34347373
Appl Environ Microbiol. 2005 Sep;71(9):4951-9
pubmed: 16151072
Crit Rev Microbiol. 2020 Aug;46(4):397-419
pubmed: 32885723
Appl Environ Microbiol. 1999 Jun;65(6):2429-38
pubmed: 10347023
Mol Microbiol. 2011 Jul;81(2):395-414
pubmed: 21564338
Microbes Environ. 2015;30(1):1-11
pubmed: 25762345
Nat Biotechnol. 2019 Dec;37(12):1513-1520
pubmed: 31792408
Environ Microbiol. 2018 Jun;20(6):2142-2159
pubmed: 29633519
Hum Genomics. 2010 Apr;4(4):271-7
pubmed: 20511140
Phytopathology. 2007 Feb;97(2):250-6
pubmed: 18944383
Appl Microbiol Biotechnol. 2013 Feb;97(3):1361-71
pubmed: 22526784
Appl Environ Microbiol. 2006 Nov;72(11):7083-90
pubmed: 17088380
ISME J. 2009 Aug;3(8):955-65
pubmed: 19421236
Ecol Lett. 2012 May;15(5):468-74
pubmed: 22394557
Phytopathology. 2001 Sep;91(9):873-81
pubmed: 18944233
Appl Environ Microbiol. 2001 Jun;67(6):2545-54
pubmed: 11375162
Front Microbiol. 2018 Aug 14;9:1867
pubmed: 30158910
J Bacteriol. 1999 May;181(10):3155-63
pubmed: 10322017
Proc Biol Sci. 2018 Dec 19;285(1893):20182035
pubmed: 30963908
Phytopathology. 2015 May;105(5):597-607
pubmed: 25710204
Sci Adv. 2019 Sep 25;5(9):eaaw0759
pubmed: 31579818
ISME J. 2009 May;3(5):549-62
pubmed: 19242532
FEMS Microbiol Ecol. 2016 Aug;92(8):
pubmed: 27199346
Annu Rev Phytopathol. 1991;29:65-87
pubmed: 18479193
Microbiologyopen. 2022 Apr;11(2):e1283
pubmed: 35478286
Nucleic Acids Res. 2017 Jul 3;45(W1):W36-W41
pubmed: 28460038
Nucleic Acids Res. 2019 Jul 2;47(W1):W81-W87
pubmed: 31032519
Appl Environ Microbiol. 1992 Jan;58(1):353-8
pubmed: 16348633