Craniotomy for acute monitoring of pial vessels in the rodent brain.
ACSF, artificial cerebrospinal fluid
BBB, blood-brain barrier
Blood-brain barrier
CNS, central nervous system
Cerebrovascular imaging
Cranial window
Craniotomy
ECs, endothelial cells
Electrocorticography
SpO2, blood oxygen saturation
Journal
MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829
Informations de publication
Date de publication:
2022
2022
Historique:
received:
24
01
2022
accepted:
02
04
2022
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
29
4
2022
Statut:
epublish
Résumé
A growing awareness for vascular contribution to pathogenesis of brain diseases increases the need for techniques that allow high-resolution imaging and quantification of changes in function and structure of cerebral microvessels. Cerebral vessels are very sensitive structures, making them vulnerable for injury. In addition, they are uniquely characterized with the blood-brain barrier, and an extra caution is required during procedures that involve engagement of cerebral vessels (i.e., craniotomy). Using state of the art facilities, including 3D intravital microscope, we describe here in details:•The steps and equipment required for drilling a craniotomy and removing of the dura, while keeping brain parenchyma and vessels intact. This enables long duration of live and direct monitoring of pial vessels and imaging of BBB permeability.•We present the craniotomy procedure that relevant and compatible with imaging pial vessels and monitoring the blood-brain barrier in small rodents.
Identifiants
pubmed: 35478597
doi: 10.1016/j.mex.2022.101694
pii: S2215-0161(22)00076-0
pmc: PMC9036111
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101694Informations de copyright
© 2022 The Author(s). Published by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Neurobiol Dis. 2010 Jan;37(1):13-25
pubmed: 19664713
In Vivo. 2007 May-Jun;21(3):471-9
pubmed: 17591356
N Engl J Med. 2001 Aug 2;345(5):311-8
pubmed: 11484687
Cerebrovasc Dis. 2008;26(6):612-7
pubmed: 18946217
J Cereb Blood Flow Metab. 2014 Nov;34(11):1791-801
pubmed: 25160672
Neuroimage. 2010 Jan 1;49(1):337-44
pubmed: 19682584
Neurobiol Dis. 2012 Dec;48(3):495-506
pubmed: 22782081
Semin Cell Dev Biol. 2015 Feb;38:2-6
pubmed: 25681530
Int J Mol Sci. 2021 Oct 28;22(21):
pubmed: 34769073
Can J Neurol Sci. 2001 May;28(2):120-4
pubmed: 11383935
Neurosurg Focus. 2009 Jun;26(6):E4
pubmed: 19485717