Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain.

Animals Diabetes Mellitus, Type 2 / metabolism Fibroblast Growth Factor 1 / pharmacology Glucose / metabolism Hypothalamus / metabolism Mice Signal Transduction
Metabolism Model Organisms Molecular Biology Neuroscience Signal Transduction

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 06 2022
Historique:
entrez: 28 4 2022
pubmed: 29 4 2022
medline: 30 4 2022
Statut: epublish

Résumé

Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).

Identifiants

DOI: 10.1016/j.xpro.2022.101329 PMID: 35479117 PMC: PMC9035806
pubmed: 35479117
doi: 10.1016/j.xpro.2022.101329
pii: S2666-1667(22)00209-X
pmc: PMC9035806
doi:

Substances chimiques

Fibroblast Growth Factor 1 104781-85-3
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101329

Subventions

Organisme : NIDDK NIH HHS
ID : R03 DK128383
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK089056
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK017047
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK083042
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK101997
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007312
Pays : United States
Organisme : NIDDK NIH HHS
ID : K08 DK114474
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK035816
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK124238
Pays : United States

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Diabetes. 2019 May;68(5):1054-1061
pubmed: 30796029
Nat Med. 2016 Jul;22(7):800-6
pubmed: 27213816
iScience. 2021 Aug 04;24(9):102944
pubmed: 34430821
Nat Commun. 2020 Sep 7;11(1):4458
pubmed: 32895383
Diabetes. 2019 Mar;68(3):654-664
pubmed: 30523024

Auteurs

Jenny M Brown (JM)

Department of Medicine, University of Washington Medicine Diabetes Institute, 750 Republican St, F770, Seattle, WA 98109, USA.
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, 2200 Copenhagen, Denmark.

Bao Anh Phan (BA)

Department of Medicine, University of Washington Medicine Diabetes Institute, 750 Republican St, F770, Seattle, WA 98109, USA.

Nadia Aalling (N)

Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, 2200 Copenhagen, Denmark.

Gregory J Morton (GJ)

Department of Medicine, University of Washington Medicine Diabetes Institute, 750 Republican St, F770, Seattle, WA 98109, USA.

Michael W Schwartz (MW)

Department of Medicine, University of Washington Medicine Diabetes Institute, 750 Republican St, F770, Seattle, WA 98109, USA.

Jarrad M Scarlett (JM)

Department of Medicine, University of Washington Medicine Diabetes Institute, 750 Republican St, F770, Seattle, WA 98109, USA.
Department of Pediatric Gastroenterology and Hepatology, Seattle Children's Hospital, Seattle, WA 98145, USA.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Combined micro-osmotic pump infusion and...
questionsmedicales.fr › Maladies endocriniennes › Diabète › Diabète de type 2 › Combined micro-osmotic pump infusion and...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Facteurs de croissance fibroblastique › Facteur de croissance fibroblastique de type 1 › Combined micro-osmotic pump infusion and...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Combined micro-osmotic pump infusion and...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Prosencéphale › Diencéphale › Hypothalamus › Combined micro-osmotic pump infusion and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Combined micro-osmotic pump infusion and...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Combined micro-osmotic pump infusion and...