Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics.
Immunology
Microbiology
Molecular Biology
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 06 2022
17 06 2022
Historique:
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
30
4
2022
Statut:
epublish
Résumé
The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).
Identifiants
pubmed: 35479118
doi: 10.1016/j.xpro.2022.101300
pii: S2666-1667(22)00180-0
pmc: PMC8938258
doi:
Substances chimiques
Nucleic Acids
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101300Subventions
Organisme : Wellcome Trust
ID : 218537/Z/19/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 213984/Z/18/Z
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N013700/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R50225X/1
Pays : United Kingdom
Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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