High-Throughput Sequencing Reveals CXCR4 and IGF1 Behave Different Roles in Weightlessness Osteoporosis.
Journal
Stem cells international
ISSN: 1687-966X
Titre abrégé: Stem Cells Int
Pays: United States
ID NLM: 101535822
Informations de publication
Date de publication:
2022
2022
Historique:
received:
03
03
2022
accepted:
24
03
2022
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
29
4
2022
Statut:
epublish
Résumé
This study is aimed at screening the differential expression profiles of mRNA under weightlessness osteoporosis through high-throughput sequencing technology, as well as investigating the pathogenesis of weightlessness osteoporosis at the molecular level especially in bone marrow mesenchymal stem cells (BMSCs). The mouse bone marrow mesenchymal stem cell line was divided into ground group and simulated microgravity (SMG) group. BMP-2 was used to induce osteogenic differentiation, and SMG group was placed into 2D-gyroscope to simulate weightless condition. Transcriptome sequencing was performed by Illumina technology, DEGs between ground and SMG group was conducted using the DEseq2 algorithm. Molecular functions and signaling pathways enriched by DEGs were then comprehensively analyzed via multiple bioinformatic approaches including but not limited to GO, KEGG, GSEA, and PPI analysis. A total of 263 DEGs were identified by comparing these 2 groups, including 186 upregulated genes and 77 downregulated genes. GO analysis showed that DEGs were enriched in osteoblasts, osteoclasts cell proliferation, differentiation, and apoptosis; KEGG analysis revealed that DEGs were significantly enriched in the TNF signaling pathway and FoxO signaling pathway; the enrichment results from Reactome database displayed that DEGs were mainly involved in the transcription of Hoxb3 gene, RUNX1 recruitment KMT2A gene, and activation of Hoxa2 chromatin signaling pathway. The four genes, IL6, CXCR4, IGF1, and PLOD2, were identified as hub genes for subsequent analysis. This study elucidated the significance of 10 hub genes in the development of weightlessness osteoporosis. In addition, the results of this study provide a theoretical basis and novel ideas for the subsequent research of the pathogenesis and clinical treatment of weightlessness osteoporosis.
Identifiants
pubmed: 35479581
doi: 10.1155/2022/5719077
pmc: PMC9038434
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5719077Informations de copyright
Copyright © 2022 Dong Wang et al.
Déclaration de conflit d'intérêts
All authors declared no conflicts of interests related to this manuscript, and all authors have approved the publication of this work.
Références
BMC Syst Biol. 2014;8 Suppl 4:S11
pubmed: 25521941
Science. 2016 Apr 8;352(6282):175-80
pubmed: 27124451
Cell Prolif. 2007 Oct;40(5):671-84
pubmed: 17877609
Sci Rep. 2015 Dec 21;5:18655
pubmed: 26686902
Nature. 2001 Feb 22;409(6823):1115-8
pubmed: 11234026
Yi Chuan. 2015 Sep;37(9):885-98
pubmed: 26399528
Bone. 2013 Nov;57(1):36-40
pubmed: 23886838
Apoptosis. 2020 Feb;25(1-2):73-91
pubmed: 31768842
Nature. 1998 Jun 11;393(6685):595-9
pubmed: 9634238
J Biol Chem. 1998 May 22;273(21):12881-6
pubmed: 9582318
Osteoarthritis Cartilage. 2014 Dec;22(12):2017-24
pubmed: 25456297
DNA Cell Biol. 2019 May;38(5):457-467
pubmed: 30864829
Endocrinol Metab Clin North Am. 2003 Mar;32(1):115-34, vii
pubmed: 12699295
Annu Rev Med. 1995;46:249-56
pubmed: 7598461
Am J Med Genet A. 2004 Dec 1;131(2):115-20
pubmed: 15523624
Aging (Albany NY). 2021 Oct 11;13(19):23284-23307
pubmed: 34633991
Aging (Albany NY). 2021 Mar 3;13(6):8248-8275
pubmed: 33686952
Zhongguo Gu Shang. 2012 Jul;25(7):611-6
pubmed: 23116001
Cancer Cell Int. 2021 Jul 14;21(1):377
pubmed: 34261456
J Med Invest. 2001 Aug;48(3-4):147-56
pubmed: 11694954
Crit Rev Oral Biol Med. 2001;12(3):252-61
pubmed: 11497376
Osteoporos Int. 1998;8 Suppl 1:S37-42
pubmed: 9682796
Stem Cells. 2001;19(3):180-92
pubmed: 11359943
Genome Biol. 2003;4(5):P3
pubmed: 12734009
Front Oncol. 2021 Oct 19;11:741403
pubmed: 34737956
J Bone Miner Res. 2015 Nov;30(11):1994-2004
pubmed: 25974843
Endocrinology. 2004 May;145(5):2421-32
pubmed: 14749352
Biomed Res Int. 2021 May 28;2021:8852574
pubmed: 34136574
Immunol Lett. 2016 Sep;177:6-15
pubmed: 27363619
J Immunol. 1990 Nov 15;145(10):3297-303
pubmed: 2121824
Endocrinology. 1991 Sep;129(3):1513-20
pubmed: 1714833
Lab Invest. 2010 Aug;90(8):1169-78
pubmed: 20368703
Biochem Res Int. 2020 Jan 24;2020:4508108
pubmed: 32047668
Br J Cancer. 2016 Jul 26;115(3):354-63
pubmed: 27310702
Nature. 2000 Nov 30;408(6812):535-6
pubmed: 11117729
Annu Rev Immunol. 2000;18:217-42
pubmed: 10837058
Clin Med (Lond). 2014 Apr;14(2):187-91
pubmed: 24715132
Ciba Found Symp. 1992;167:5-16; discussion 16-23
pubmed: 1425018