Montmorillonite stabilized chitosan-
Journal
RSC advances
ISSN: 2046-2069
Titre abrégé: RSC Adv
Pays: England
ID NLM: 101581657
Informations de publication
Date de publication:
06 Sep 2021
06 Sep 2021
Historique:
received:
21
06
2021
accepted:
06
09
2021
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
29
4
2022
Statut:
epublish
Résumé
The role of polymers has played a crucial role in developing templates that can promote regeneration as tissue-engineered matrices. The present study aims to develop functional matrices involving the protein mucin. The mucin used in this study is characterised using MALDI-TOF TOF and CD spectroscopy prior to conjugation. Thereupon, a hybrid scaffold comprising of a polysaccharide, chitosan, chemically conjugated to a protein, mucin, and encapsulated with montmorillonite is developed. Grafting of hydroxyethyl methacrylate (HEMA) is done to overcome the issue of mechanical weakness that mucin hydrogels usually undergo. It was observed that the presence of montmorillonite led to the stability of the hydrogels. The conjugations with varied ratios of the polysaccharide and protein were characterized using spectroscopic techniques. The prepared gels showed appreciable material properties in terms of water uptake and porosity. Hydrogels with different ratios of the polysaccharide and protein were evaluated for their biocompatibility. The biological evaluation of the hydrogels was performed with MC3T3E1 and C2C12 cell lines indicating their potential for wider tissue engineering applications.
Identifiants
pubmed: 35480259
doi: 10.1039/d1ra04803a
pii: d1ra04803a
pmc: PMC9041129
doi:
Types de publication
Journal Article
Langues
eng
Pagination
30329-30342Informations de copyright
This journal is © The Royal Society of Chemistry.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
Biophys J. 2016 Dec 6;111(11):2377-2386
pubmed: 27926839
J Chem Theory Comput. 2008 Dec 9;4(12):2141-9
pubmed: 26620485
Bone Res. 2017 May 30;5:17014
pubmed: 28584674
Macromol Biosci. 2021 Jun;21(6):e2000381
pubmed: 33871165
J Mater Sci Mater Med. 2009 Dec;20(12):2527-40
pubmed: 19629650
J Biomed Mater Res A. 2013 Sep;101(9):2644-60
pubmed: 23413041
Carbohydr Polym. 2016 Nov 5;152:129-139
pubmed: 27516257
Annu Rev Physiol. 1995;57:635-57
pubmed: 7778881
ACS Biomater Sci Eng. 2019 Oct 14;5(10):4994-5004
pubmed: 33455247
Adv Healthc Mater. 2021 May;10(10):e2002152
pubmed: 33644996
Methods Mol Biol. 2012;800:227-40
pubmed: 21964792
J Pept Res. 1999 Oct;54(4):290-310
pubmed: 10532235
Sci Pharm. 2015 Oct 22;84(4):603-617
pubmed: 28656939
RSC Adv. 2018 Nov 27;8(69):39567-39578
pubmed: 35558028
Tissue Eng Part B Rev. 2010 Dec;16(6):617-27
pubmed: 20836752
Anal Chem. 2016 Dec 6;88(23):11609-11615
pubmed: 27791356
ACS Appl Mater Interfaces. 2017 Mar 22;9(11):9265-9270
pubmed: 28282110
Cell Biol Int. 2014 Jan;38(1):72-84
pubmed: 24108671
Mater Today Bio. 2019 Sep;4:100028
pubmed: 31853520
Langmuir. 2005 Aug 30;21(18):8344-53
pubmed: 16114941
Crit Rev Biochem Mol Biol. 1992;27(1-2):57-92
pubmed: 1727693
RSC Adv. 2020 Oct 6;10(60):36751-36777
pubmed: 35517957
J Mater Chem B. 2015 May 21;3(19):4052-4062
pubmed: 32262627
Biomed Mater. 2008 Sep;3(3):034122
pubmed: 18765898
J Biol Chem. 1987 Aug 15;262(23):11339-44
pubmed: 3611111
Carbohydr Polym. 2018 Dec 1;201:490-499
pubmed: 30241846
J Control Release. 2016 Oct 28;240:109-126
pubmed: 26571000
Int J Pharm. 2018 Dec 1;552(1-2):301-311
pubmed: 30291961
Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:1036-1051
pubmed: 30678895
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111720
pubmed: 33545871
Mater Sci Eng C Mater Biol Appl. 2018 Aug 1;89:8-14
pubmed: 29752122
Colloids Surf B Biointerfaces. 2014 Oct 1;122:175-183
pubmed: 25033437
Langmuir. 2015 Aug 4;31(30):8303-9
pubmed: 26153254
Saudi Pharm J. 2012 Jul;20(3):263-71
pubmed: 23960799
J Tissue Eng Regen Med. 2017 May;11(5):1500-1513
pubmed: 26076675
Nat Commun. 2019 Aug 6;10(1):3523
pubmed: 31388014
Mater Sci Eng C Mater Biol Appl. 2015 Aug;53:212-21
pubmed: 26042709
Biomimetics (Basel). 2019 Apr 25;4(2):
pubmed: 31105217
J Biomed Mater Res A. 2015 Jun;103(6):2077-101
pubmed: 25331212
J Colloid Interface Sci. 2014 Jun 15;424:113-9
pubmed: 24767506
Biomacromolecules. 2014 Jul 14;15(7):2725-34
pubmed: 24955658
Biochim Biophys Acta. 2005 Aug 10;1751(2):119-39
pubmed: 16027053
Prep Biochem Biotechnol. 2015;45(1):84-99
pubmed: 24547990
Carbohydr Polym. 2018 Jun 1;189:87-98
pubmed: 29580430
J Phys Chem B. 2010 Aug 19;114(32):10666-73
pubmed: 20734475
Acta Biomater. 2011 Aug;7(8):3036-41
pubmed: 21549864
J Transl Med. 2016 Sep 20;14:271
pubmed: 27645770