Desiccation-tolerance and globular proteins adsorb similar amounts of water.
desiccation
disordered proteins
tardigrades
thermogravimetric analysis
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
revised:
11
02
2022
received:
14
12
2021
accepted:
15
02
2022
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
3
5
2022
Statut:
ppublish
Résumé
When exposed to desiccation stress, extremotolerant organisms from all domains of life produce protective disordered proteins with the potential to inform the design of excipients for formulating biologics and industrial enzymes. However, the mechanism(s) of desiccation protection remain largely unknown. To investigate the role of water sorption in desiccation protection, we use thermogravimetric analysis to study water adsorption by two desiccation-tolerance proteins, cytosolic abundant heat soluble protein D from tardigrades and late embryogenesis abundant protein 4 from the anhydrobiotic midge Polypedilum vanderplanki, and, as a control, the globular B1 domain of staphylococcal protein G. All samples adsorb similar amounts of water, suggesting that modulated water retention is not responsible for dehydration protection by desiccation-tolerance proteins.
Identifiants
pubmed: 35481639
doi: 10.1002/pro.4288
pmc: PMC8994485
doi:
Substances chimiques
Proteins
0
Water
059QF0KO0R
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4288Subventions
Organisme : NIH HHS
ID : R01GM127291
Pays : United States
Informations de copyright
© 2022 The Protein Society.
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