The mycobacterial desaturase DesA2 is associated with mycolic acid biosynthesis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 04 2022
28 04 2022
Historique:
received:
07
09
2021
accepted:
11
04
2022
entrez:
28
4
2022
pubmed:
29
4
2022
medline:
3
5
2022
Statut:
epublish
Résumé
Mycolic acids are critical for the survival and virulence of Mycobacterium tuberculosis, the causative agent of tuberculosis. Double bond formation in the merochain of mycolic acids remains poorly understood, though we have previously shown desA1, encoding an aerobic desaturase, is involved in mycolic acid desaturation. Here we show that a second desaturase encoded by desA2 is also involved in mycolate biosynthesis. DesA2 is essential for growth of the fast-growing Mycobacterium smegmatis in laboratory media. Conditional depletion of DesA2 led to a decrease in mycolic acid biosynthesis and loss of mycobacterial viability. Additionally, DesA2-depleted cells also accumulated fatty acids of chain lengths C
Identifiants
pubmed: 35484172
doi: 10.1038/s41598-022-10589-y
pii: 10.1038/s41598-022-10589-y
pmc: PMC9050676
doi:
Substances chimiques
Mycolic Acids
0
Fatty Acid Desaturases
EC 1.14.19.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6943Subventions
Organisme : Medical Research Council
ID : MR/K00042X/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N01314X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S000542/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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