Daily longitudinal sampling of SARS-CoV-2 infection reveals substantial heterogeneity in infectiousness.


Journal

Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869

Informations de publication

Date de publication:
05 2022
Historique:
received: 06 08 2021
accepted: 15 03 2022
pubmed: 29 4 2022
medline: 6 5 2022
entrez: 28 4 2022
Statut: ppublish

Résumé

The dynamics of SARS-CoV-2 replication and shedding in humans remain poorly understood. We captured the dynamics of infectious virus and viral RNA shedding during acute infection through daily longitudinal sampling of 60 individuals for up to 14 days. By fitting mechanistic models, we directly estimated viral expansion and clearance rates and overall infectiousness for each individual. Significant person-to-person variation in infectious virus shedding suggests that individual-level heterogeneity in viral dynamics contributes to 'superspreading'. Viral genome loads often peaked days earlier in saliva than in nasal swabs, indicating strong tissue compartmentalization and suggesting that saliva may serve as a superior sampling site for early detection of infection. Viral loads and clearance kinetics of Alpha (B.1.1.7) and previously circulating non-variant-of-concern viruses were mostly indistinguishable, indicating that the enhanced transmissibility of this variant cannot be explained simply by higher viral loads or delayed clearance. These results provide a high-resolution portrait of SARS-CoV-2 infection dynamics and implicate individual-level heterogeneity in infectiousness in superspreading.

Identifiants

pubmed: 35484231
doi: 10.1038/s41564-022-01105-z
pii: 10.1038/s41564-022-01105-z
pmc: PMC9084242
mid: NIHMS1789713
doi:

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

640-652

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI152703
Pays : United States
Organisme : NHLBI NIH HHS
ID : U54 HL143541
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI139246
Pays : United States

Commentaires et corrections

Type : UpdateOf

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer Nature Limited.

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Auteurs

Ruian Ke (R)

T-6, Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM, USA.

Pamela P Martinez (PP)

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Department of Statistics, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Rebecca L Smith (RL)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Laura L Gibson (LL)

Division of Infectious Diseases and Immunology, Departments of Medicine and Pediatrics, University of Massachusetts Medical School, Worcester, MA, USA.

Agha Mirza (A)

Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Madison Conte (M)

Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Nicholas Gallagher (N)

Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Chun Huai Luo (CH)

Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Junko Jarrett (J)

Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Ruifeng Zhou (R)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Abigail Conte (A)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Tongyu Liu (T)

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Mireille Farjo (M)

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Kimberly K O Walden (KKO)

High-Performance Biological Computing at the Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Gloria Rendon (G)

High-Performance Biological Computing at the Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Christopher J Fields (CJ)

High-Performance Biological Computing at the Roy J. Carver Biotechnology Center, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Leyi Wang (L)

Veterinary Diagnostic Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Richard Fredrickson (R)

Veterinary Diagnostic Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Darci C Edmonson (DC)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Melinda E Baughman (ME)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Karen K Chiu (KK)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Hannah Choi (H)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Kevin R Scardina (KR)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Shannon Bradley (S)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Stacy L Gloss (SL)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Crystal Reinhart (C)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Jagadeesh Yedetore (J)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Jessica Quicksall (J)

Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Alyssa N Owens (AN)

Center for Clinical and Translational Research, University of Massachusetts Medical School, Worcester, MA, USA.

John Broach (J)

UMass Memorial Medical Center, Worcester, MA, USA.
Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, MA, USA.

Bruce Barton (B)

Division of Biostatistics and Health Services Research, University of Massachusetts Medical School, Worcester, MA, USA.
Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, MA, USA.

Peter Lazar (P)

Division of Biostatistics and Health Services Research, University of Massachusetts Medical School, Worcester, MA, USA.

William J Heetderks (WJ)

National Institute for Biomedical Imaging and Bioengineering, Bethesda, MD, USA.

Matthew L Robinson (ML)

Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Heba H Mostafa (HH)

Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Yukari C Manabe (YC)

Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD, USA.
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Andrew Pekosz (A)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

David D McManus (DD)

Division of Cardiology, University of Massachusetts Medical School, Worcester, MA, USA.

Christopher B Brooke (CB)

Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA. cbrooke@illinois.edu.
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA. cbrooke@illinois.edu.

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