Isolation, Structural Analysis and Anti-Inflammatory Activity of a Polysaccharide from Ilex cornuta Fruits.
Ilex cornuta fruits
anti-inflammatory activity
isolation
polysaccharide
structural characterization
Journal
Chemistry & biodiversity
ISSN: 1612-1880
Titre abrégé: Chem Biodivers
Pays: Switzerland
ID NLM: 101197449
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
26
01
2022
accepted:
07
04
2022
pubmed:
30
4
2022
medline:
23
6
2022
entrez:
29
4
2022
Statut:
ppublish
Résumé
In the present study, a polysaccharide from Ilex cornuta fruits (LCFP-3) was obtained by hot water extraction, Diethyaminoethyl cellulose-52 (DEAE-52) chromatography column and Sephadex G-100 gel column purification. Its structural characteristics were further explored using high performance anion exchange chromatography (HPAEC), gas chromatography and mass spectrometry (GC/MS), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. Monosaccharide composition analysis revealed LCFP-3 contained mainly Galactose (31.92 %), Arabinose (25.87 %) and Galacturonic acid (23.35 %) while small percentage of Rhamnose, Glucose, Mannose and Xylose. Chemical composition analysis showed that the total sugar content of LCFP-3 was 90.31 % and the protein content was 0.246 %. Gel permeation chromatography (GPC) analysis showed that its average molecular weight was 41.199 kDa. Structural analysis showed that LCFP-3 may be composed of residues, T-α-Arap, T-α-Rhap, 1,3-α-Arap, 1,4-α-Arap, T-β-Galp, 1,4-α-GalpA(OMe), 1,4-β-Glcp, 1,3-β-Galp, 1,3,6-β-Manp, 1,6-β-Galp, 1,3,4-β-GalpA, 1,4,6-β-Manp, 1,3,6-β-Glcp, 1,2,3,4-α-Xylp. The anti-inflammatory activity of LCFP-3 was evaluated using lipopolysaccharide (LPS)-induced RAW246.7 macrophages. The results showed that 1-200 μg/mL LCFP-3 could dose-dependently protect against LPS-induced toxicity and 1 μg/mL LCFP-3 could significantly inhibit LPS-induced NO production. Therefore, LCFP-3 exerted an anti-inflammatory activity and has great potential as a functional ingredient.
Identifiants
pubmed: 35484695
doi: 10.1002/cbdv.202200084
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Lipopolysaccharides
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200084Subventions
Organisme : National Natural Science Foundation of China
ID : 81572989
Informations de copyright
© 2022 Wiley-VHCA AG, Zurich, Switzerland.
Références
M. Cui, Y. Wang, J. Elango, J. Wu, K. Liu, Y. Jin, ‘Cereus sinensis polysaccharide alleviates antibiotic-associated diarrhea based on modulating the gut microbiota in C57BL/6 mice’,
Front. Nutr. Sci. 2021, 8, 751992.
M. Cui, M. Zhang, K. Liu, ‘Colon-targeted drug delivery of polysaccharide-based nanocarriers for synergistic treatment of inflammatory bowel disease: A review’, Carbohydr. Polym. 2021, 272, 118530.
W. Zhang, S. Dang, G. Zhang, H. He, X. Wen, ‘Genetic polymorphisms of IL-10, IL-18 and IL12B are associated with risk of non-small cell lung cancer in a Chinese Han population’, Int. Immunopharmacol. 2019, 77, 105938.
M. Cui, J. Wu, S. Wang, H. Shu, M. Zhang, K. Liu, K. Liu, ‘Characterization and anti-inflammatory effects of sulfated polysaccharide from the red seaweed Gelidium pacificum Okamura’, Int. J. Biol. Macromol. 2019, 129, 377-385.
S. Bindu, U. Mazumder S Bandyopadhyay, ‘Non-steroidal anti-inflammatory drugs (NSAIDs) and organ damage: A current perspective’, Biochem. Pharmacol. 2020, 180, 114147.
W. B. Reid-Varley, C. Ponce Martinez, K. A. Khurshid, ‘Sleep disorders and disrupted sleep in addiction, withdrawal and abstinence with focus on alcohol and opioids’, J. Neurol. Sci. 2020, 411, 116713.
B. M. Shah, S. S. Palakurthi, T. Khare, S. Khare, S. Palakurthi, ‘Natural proteins and polysaccharides in the development of micro/nano delivery systems for the treatment of inflammatory bowel disease’, Int. J. Biol. Macromol. 2020, 165 (Pt. A), 722-737.
S. Zhou, G. Huang, Preparation, ‘Structure and activity of polysaccharide phosphate esters’, Biomed. Pharmacother. 2021, 144, 112332.
S. Zhou, G. Huang, G. Chen, ‘Extraction, structural analysis, derivatization and antioxidant activity of polysaccharide from Chinese yam’, Food Chem. 2021, 361, 130089.
G. Benchamas, G. Huang, S. Huang, H. Huang, ‘Preparation and biological activities of chitosan oligosaccharides’, Trends Food Sci. Technol. 2021, 107, 38-44.
W. Yang, G. Huang, F. Chen, H. Huang, ‘Extraction/synthesis and biological activities of selenopolysaccharide’, Trends Food Sci. Technol. 2021, 109, 211-218.
G. Huang, F. Chen, W. Yang, H. Huang, ‘Preparation, deproteinization and comparison of bioactive polysaccharides’, Trends Food Sci. Technol. 2021, 109, 564-568.
W. Yang, G. Huang, ‘Extraction methods and activities of natural glucans’, Trends Food Sci. Technol. 2021, 112, 50-57.
F. Chen, G. Huang, H. Huang, ‘Preparation, analysis, antioxidant activities in vivo of phosphorylated polysaccharide from Momordica charantia’, Carbohydr. Polym. 2021, 252, 117179.
F. Chen, S. Huang, G. Huang, ‘Preparation, activity, and antioxidant mechanism of rice bran polysaccharide’, Food Funct. 2021, 12, 834.
J. Li, G. Huang, ‘Extraction, purification, separation, structure, derivatization and activities of polysaccharide from Chinese date’, Process Biochem. 2021, 110, 231-242.
B. F. Holanda, D. Freitas de Araujo, J. N. R. Silva, M. G. Pereira, A. F. Pires, A. M. S. Assreuy, ‘Polysaccaride-rich extract of Caesalpina ferrea stem barks attenuates mice acute inflammation induced by zymosan: Oxidative stress modulation’, J. Ethnopharmacol. 2020, 267, 113501.
D. Mao, X. Y. Tian, D. Mao, S. W. Hung, C. C. Wang, C. B. S. Lau, H. M. Lee, C. K. Wong, E. Chow, X. Ming, H. Cao, R. C. Ma, P. K. S. Chan, A. P. S. Kong, J. J. X. Li, G. A. Rutter, W. H. Tam, J. C. N. Chan, ‘A polysaccharide extract from the medicinal plant Maidong inhibits the IKK-NF-κB pathway and IL-1β-induced islet inflammation and increases insulin secretion’, J. Biol. Chem. 2020, 295, 12573-12587.
J. Qu, P. L. Huang, L. Zhang, Y. Qiu, H. Qi, A. Leng, D. Shang, ‘Hepatoprotective effect of plant polysaccharides from natural resources: A review of the mechanisms and structure-activity relationship’, Int. J. Biol. Macromol. 2020, 161, 24-34.
Y. Xu, H. Jiang, C. Sun, M. Adu-Frimpong, W. Deng, J. Yu, X. Xu, ‘Antioxidant and hepatoprotective effects of purified Rhodiola rosea polysaccharides’, Int. J. Biol. Macromol. 2018, 117, 167-178.
F. Yi, X. Zhao, Y. Peng, P. G. Xiao, ‘Genus llex L.: phytochemistry, ethnopharmacology, and pharmacology’, Chin. Herb. Med. 2016, 8, 209-230.
S. S. Li, Y. L. Liu, Q. M. Xu, C. M. Mao, S. L. Yang, ‘Three new triterpenoids isolated from the aerial parts of Ilex cornuta and protective effects against H2O2-induced myocardial cell injury’, Chin. J. Nat. Med. 2017, 15, 115-120.
Y. Zhang, X. Pan, S. Ran, K. Wang, ‘Purification, structural elucidation and anti-inflammatory activity in vitro of polysaccharides from Smilax china L’, Int. J. Biol. Macromol. 2019, 139, 233-243.
R. Chen, H. Li, S. Li, C. Jin, J. Lu, ‘Extraction optimization, preliminary characterization and immunological activity of polysaccharides from figs’, Int. J. Biol. Macromol. 2015, 72, 185-194.
H. Tian, H. Liu, W. Song, L. Zhu, T. Zhang, R. Li, X. Yin, ‘Structure, antioxidant and immunostimulatory activities of the polysaccharides from Sargassum carpophyllum’, Algal Res. 2020, 49, 101853.
R. Zhou, M. Cui, Y. Wang, M. Zhang, F. Li, K. Liu, ‘Isolation, structure identification and anti-inflammatory activity of a polysaccharide from Phragmites rhizoma’, Int. J. Biol. Macromol. 2020, 161, 810-817.
M. Cui, M. Zhang, J. Wu, P. Han, M. Lv, L. Dong, K. Liu, ‘Marine polysaccharides from Gelidium pacificum Okamura and Cereus sinensis reveal prebiotic functions’, Int. J. Biol. Macromol. 2020, 164, 4381-4390.
Y. Liu, X. Duan, M. Zhang, C. Li, Z. Zhang, B. Hu, A. Liu, Q. Li, H. Chen, Z. Tang, W. Wu, D. Chen, ‘Extraction, structure characterization, carboxymethylation and antioxidant activity of acidic polysaccharides from Craterellus cornucopioides’, Ind. Crops Prod. 2021, 159, 113079.
F. Li, Y. Wei, L. Liang, L. Huang, G. Yu, Q. Li, ‘A novel low-molecular-mass pumpkin polysaccharide Structural characterization, antioxidant activity, and hypoglycemic potential’, Carbohydr. Polym. 2021, 251, 117090.
H. Hao, Y. Han, L. Yang, L. Hu, X. Duan, X. Yang, R. Huang, ‘Structural characterization and immunostimulatory activity of a novel polysaccharide from green alga Caulerpa racemosa var peltata’, Int. J. Biol. Macromol. 2019, 134, 891-900.
F. Zhang, X. Zhang, S. Guo, F. Cao, X. Zhang, Y. Wang, J. Liu, B. Qian, Y. Yan, P. Chen, C. Xu, C. Liu, D. Qian, J. A. Duan, ‘An acidic heteropolysaccharide from Lycii fructus: Purification, characterization, neurotrophic and neuroprotective activities in vitro’, Carbohydr. Polym. 2020, 249, 116894.
Z. Li, L. An, S. Zhang, Z. Shi, J. Bao, M. Tuerhong, M. Abudukeremu, J. Xu, Y. Guo, ‘Structural elucidation and immunomodulatory evaluation of a polysaccharide from Stevia rebaudiana leaves’, Food Chem. 2021, 364.
J. Wu, T. Chen, F. Wan, J. Wang, X. Li, W. Li, L. Ma, ‘Structural characterization of a polysaccharide from Lycium barbarum and its neuroprotective effect against β-amyloid peptide neurotoxicity’, Int. J. Biol. Macromol. 2021, 176, 352-363.
H. Zhang, P. Zou, H. Zhao, J. Qiu, J. M. Regenstein, X. Yang, ‘Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis’, Carbohydr. Polym. 2021, 251, 117078.
D. Xu, C. Wang, Z. Zhuo, M. Ye, B. Pu, ‘Extraction, purification and antioxidant activity of polysaccharide from cold pressed oil cake of ‘Tengjiao’ seed’, Int. J. Biol. Macromol. 2020, 163, 508-518.
R. Lobo, Prabhu, K. S. Shirwaikar, M. A. Ballal, C. Balachandran, A. Shirwaikar, ‘A HPTLC densitometric method for the determination of aloeverose in Aloe vera gel’, Fitoterapia. 2010, 81, 231-233.
X. Yang, F. Chen, G. Huang, ‘Extraction and analysis of polysaccharide from Momordica charantia’, Ind. Crops Prod. 2020, 153, 112588.
Y. Tsukakoshi, S. I. N. Naito, A. Yasui, ‘Variation in moisture, total sugar, and carotene content of Japanese carrots: Use in sample size determination’, J. Food Compos. Anal. 2009, 22, 373-380.
T. S. J. d. Silva, A. A. Soares, T. M. Rocha, A. T. Á. Pimenta, D. Miron, R. J. F. Silva, G. S. B. Viana, L. K. A. M. Leal, ‘Spondias mombin: Quality control and anti-inflammatory activity in human neutrophils’, J. Herb. Med. 2020, 24, 100393.
G. Zhan, R. Miao, F. Zhang, Y. Hao, Y. Zhang, Y. Zhang, M. Khurm, X. Zhang, Z. Guo, ‘Peraksine derivatives with potential anti-inflammatory activities from the stems of Rauvolfia vomitoria’, Fitoterapia. 2020, 146, 104704.