The HOXC-AS2/miR-876-5p/HKDC1 axis regulates endometrial cancer progression in a high glucose-related tumor microenvironment.


Journal

Cancer science
ISSN: 1349-7006
Titre abrégé: Cancer Sci
Pays: England
ID NLM: 101168776

Informations de publication

Date de publication:
Jul 2022
Historique:
revised: 22 04 2022
received: 12 12 2021
accepted: 25 04 2022
pubmed: 30 4 2022
medline: 15 7 2022
entrez: 29 4 2022
Statut: ppublish

Résumé

The tumor microenvironment (TME) is related to chronic inflammation and is currently identified as a risk factor for the occurrence and development of endometrial cancer (EC). Pyroptosis is a new proinflammatory form of programmed cell death that plays a critical role in the progression of multiple diseases. However, the important role of pyroptosis in high-glucose (HG)-related EC and the underlying molecular mechanisms remain elusive. In the present study, transcriptome high-throughput sequencing revealed significantly higher hexokinase domain-containing 1 (HKDC1) expression in EC patients with diabetes than in EC patients with normal glucose. Mechanistically, HKDC1 regulates HG-induced cell pyroptosis by modulating the production of reactive oxygen species and pyroptosis-induced cytokine release in EC. In addition, HKDC1 regulates TME formation by enhancing glycolysis, promoting a metabolic advantage in lactate-rich environments to further accelerate EC progression. Subsequently, miR-876-5p was predicted to target the HKDC1 mRNA, and HOXC-AS2 was identified to potentially inhibit the miR-876-5p/HKDC1 axis in regulating cell pyroptosis in HG-related EC. Collectively, we elucidated the regulatory role of the HOXC-AS2/miR-876-5p/HKDC1 signal transduction axis in EC cell pyroptosis at the molecular level, which may provide an effective therapeutic target for patients with diabetes who are diagnosed with EC.

Identifiants

pubmed: 35485648
doi: 10.1111/cas.15384
pmc: PMC9277262
doi:

Substances chimiques

MIRN876 microRNA, human 0
MicroRNAs 0
RNA, Long Noncoding 0
HKDC1 protein, human EC 2.7.1.1
Hexokinase EC 2.7.1.1
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2297-2310

Subventions

Organisme : Taishan Scholars
ID : ts201511073
Organisme : Shenzhen Healthcare Research Project
ID : SZLY2017030
Organisme : Special Fund for Scientific Talents of the First Affiliated Hospital of Xiamen University
ID : ZLYY201906
Organisme : Sanming Project of Medicine in Shenzhen
ID : SZSM201812075
Organisme : Xiamen Medical and Health Guiding Project Fund Project
ID : 3502Z20214ZD1020
Organisme : National Natural Science Foundation of China
ID : NSFC81672591
Organisme : National Major Scientific and Technological Special Project for "Significant New Drugs Development"
ID : 2020ZX09201005
Organisme : Fujian Natural Science Foundation (Youth Innovation) Project
ID : 2020J05308

Informations de copyright

© 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

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Auteurs

Jing Guo (J)

Department of Medical Oncology, Xiamen Key Laboratory of Antitumor Drug Transformation Research, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Feng Ye (F)

Department of Medical Oncology, Xiamen Key Laboratory of Antitumor Drug Transformation Research, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Wenli Xie (W)

Department of Gynecology, The Second Hospital of Shandong University, Jinan, Shandong, China.

Xinxin Zhang (X)

Department of Gynecologic Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong, China.

Ru Zeng (R)

Department of Medical Oncology, Xiamen Key Laboratory of Antitumor Drug Transformation Research, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Wang Sheng (W)

Department of Medical Oncology, Xiamen Key Laboratory of Antitumor Drug Transformation Research, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Yanjun Mi (Y)

Department of Medical Oncology, Xiamen Key Laboratory of Antitumor Drug Transformation Research, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China.

Xiugui Sheng (X)

Cancer Hospital of Chinese Academy of Medical Sciences, Shenzhen Center, Shenzhen, Guangdong, China.

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Classifications MeSH