The polyene antifungal candicidin is selectively packaged into membrane vesicles in Streptomyces S4.
Antifungal
Antimycin
Candicidin
Gram-positive
Membrane vesicles
Streptomyces
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
30 Apr 2022
30 Apr 2022
Historique:
received:
14
12
2021
accepted:
06
04
2022
revised:
16
03
2022
entrez:
29
4
2022
pubmed:
30
4
2022
medline:
4
5
2022
Statut:
epublish
Résumé
In recent years, much attention has been focused on the biogenesis, engineering and utilisation of outer membrane vesicles (OMVs) in Gram-negative bacteria in a range of environments and niches. While the precise mechanism of biogenesis is unknown, it is focused on the modification of the Gram-negative cell wall to facilitate blebbing at sites of weakness in and around the characteristically thin peptidoglycan layer within the periplasm. Here, we investigate the biogenesis of membrane vesicles (MVs) in the Gram-positive organism Streptomyces albus S4 (Seipke et al. J Bacteriol 193:4270-4271, 2011 and Fazal et al. Antonie Van Leeuwenhoek 113:511-520, 2020). The S. albus S4 strain is an antifungal (candicidin and antimycin) producing organism that was isolated from attine ants (Barke et al. BMC Biol 8:109, 2010). The biogenesis and characterisation of S. albus S4 MVs is demonstrated using the wild-type (WT) and mutant strains ΔantC (no antimycin production) ΔfscC (no candicidin production) and ΔantC ΔfscC (produces neither antimycin nor candicidin). Here, we have shown that the S. albus S4 strain produces MVs and that these are comprised of both specific protein profiles and secondary metabolites, with a clear demonstration of the ability to selectively package one antifungal (candicidin) but not the other (antimycin).
Identifiants
pubmed: 35488016
doi: 10.1007/s00203-022-02906-w
pii: 10.1007/s00203-022-02906-w
pmc: PMC9054904
doi:
Substances chimiques
Antifungal Agents
0
Polyenes
0
Candicidin
1403-17-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
289Informations de copyright
© 2022. The Author(s).
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