Screening of microRNA and mRNA related to secondary hair follicle morphogenesis and development and functional analysis in cashmere goats.
Cashmere goat
Functional analysis
Secondary hair follicle
mRNA
microRNA
Journal
Functional & integrative genomics
ISSN: 1438-7948
Titre abrégé: Funct Integr Genomics
Pays: Germany
ID NLM: 100939343
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
received:
28
04
2021
accepted:
07
03
2022
revised:
30
01
2022
pubmed:
30
4
2022
medline:
13
10
2022
entrez:
29
4
2022
Statut:
ppublish
Résumé
microRNA (miRNA) is a type of endogenous short-chain non-coding RNA with regulatory function found in eukaryotes, which is involved in the regulation of a variety of cellular and biological processes. However, the research on the development of cashmere goat secondary hair follicles is still relatively scarce. In this study, small RNA libraries and mRNA libraries of 45 days, 55 days, 65 days, and 75 days of fetal skin of cashmere goats were constructed, and the constructed libraries were sequenced using Illumina Hiseq4000, and the expression profiles of miRNA and mRNA in cashmere goat fetal skin were obtained. The differentially expressed miRNAs and mRNAs in six control groups were identified and the qRT-PCR experiment shows that the sequencing results are accurate. Sixty-six miRNAs related to secondary hair follicle development were screened, and used TargetScan and miRanda to predict 33 highly expressed miRNA target genes. At the same time, 664 mRNAs related to the development of secondary hair follicles were screened, and GO enrichment and KEGG pathway analysis were performed. It was found that some miRNA target genes were consistent with the screening results of mRNAs related to secondary hair follicle development and were enriched in Notch signaling pathway, TGF-β signaling pathway. Therefore, miR-145-5p-DLL4, miR-27b-3p-DLL4, miR-30e-5p-DLL4, miR-193b-3p-TGF-β1, miR-181b-5p-NOTCH2, and miR-103-3p-NOTCH2 regulatory network related to the development of secondary hair follicles were constructed and the results of dual-luciferase reporter gene assay indicated that there is a targeted relationship between chi-miR-30e-5p and DLL4, which will provide a basis for molecular mechanism of miRNA-mRNA in the development of the hair follicles in cashmere goats.
Identifiants
pubmed: 35488101
doi: 10.1007/s10142-022-00842-y
pii: 10.1007/s10142-022-00842-y
pmc: PMC9550687
doi:
Substances chimiques
MicroRNAs
0
RNA, Messenger
0
Transforming Growth Factor beta1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
835-848Subventions
Organisme : National Natural Science Foundation of China
ID : 31860627
Organisme : Plan Project of Scienceand Technology in Inner Mongolia
ID : 2019GG243
Informations de copyright
© 2022. The Author(s).
Références
Bioessays. 2005 Mar;27(3):247-61
pubmed: 15714560
Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):8108-12
pubmed: 9653148
Development. 1998 Feb;125(4):767-75
pubmed: 9435296
BMC Genomics. 2020 Jun 26;21(1):430
pubmed: 32586272
Gene. 2013 Apr 25;519(1):77-81
pubmed: 23384715
Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14653-8
pubmed: 16195376
Cell. 1993 May 21;73(4):725-34
pubmed: 8500166
Miner Electrolyte Metab. 1998;24(2-3):111-9
pubmed: 9525693
PLoS One. 2012;7(12):e50001
pubmed: 23236360
Annu Rev Biomed Eng. 2010 Aug 15;12:1-27
pubmed: 20415587
Funct Integr Genomics. 2018 Nov;18(6):701-707
pubmed: 29915875
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
G3 (Bethesda). 2021 Jan 18;11(1):
pubmed: 33561234
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Pharmaceuticals (Basel). 2011 Jun 1;4(6):848-879
pubmed: 21966254
Cell Cycle. 2021 Jan;20(2):204-210
pubmed: 33427027
Development. 2000 Jun;127(11):2421-32
pubmed: 10804183
Nat Biotechnol. 2015 Mar;33(3):290-5
pubmed: 25690850
Cytokine Growth Factor Rev. 2003 Dec;14(6):537-50
pubmed: 14563355
Annu Rev Biochem. 2010;79:351-79
pubmed: 20533884
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Nat Rev Mol Cell Biol. 2002 Oct;3(10):753-66
pubmed: 12360192
Biochem Biophys Res Commun. 2013 Aug 30;438(3):551-6
pubmed: 23916701
Nat Biotechnol. 2015 Mar;33(3):243-6
pubmed: 25748911
BMC Genomics. 2013 Jul 28;14:511
pubmed: 23889850
Annu Rev Med. 2009;60:167-79
pubmed: 19630570
Dev Biol. 1999 Aug 15;212(2):278-89
pubmed: 10433821
Sci Rep. 2020 Mar 11;10(1):4519
pubmed: 32161290
PLoS One. 2007 Jul 11;2(7):e610
pubmed: 17622355
FASEB J. 2000 Apr;14(5):752-60
pubmed: 10744631
J Invest Dermatol. 2002 Feb;118(2):216-25
pubmed: 11841536
Cell. 2008 Aug 8;134(3):521-33
pubmed: 18692474
J Investig Dermatol Symp Proc. 2003 Jun;8(1):65-8
pubmed: 12894996
Cell. 2000 Oct 13;103(2):295-309
pubmed: 11057902