A scalable hyperthermic intravesical chemotherapy (HIVEC) setup for rat models of bladder cancer.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 04 2022
29 04 2022
Historique:
received:
12
10
2021
accepted:
24
03
2022
entrez:
29
4
2022
pubmed:
30
4
2022
medline:
4
5
2022
Statut:
epublish
Résumé
Hyperthermic intravesical chemotherapy (HIVEC)-whereby the bladder is heated to ± 43 °C during a chemotherapy instillation-can improve outcomes of non-muscle invasive bladder cancer (NMIBC) treatments. Experiments in animal models are required to explore new hyperthermia based treatments. Existing HIVEC devices are not suitable for rodents or large-scale animal trials. We present a HIVEC setup compatible with orthotopic rat models. An externally heated chemotherapeutic solution is circulated in the bladder through a double-lumen catheter with flow rates controlled using a peristaltic pump. Temperature sensors in the inflow channel, bladder and outflow channel allow temperature monitoring and adjustments in real-time. At a constant flow rate of 2.5 mL/min the system rapidly reaches the desired bladder temperature of 42-43 °C with minimal variability throughout a one-hour treatment in a rat bladder phantom, as well as in euthanised and live rats. Mean intraluminal bladder temperatures were 42.92 °C (SD = 0.15 °C), 42.45 °C (SD = 0.37 °C) and 42.52 °C (SD = 0.09 °C) in the bladder phantom, euthanised, and live rats respectively. Thermal camera measurements showed homogenous heat distributions over the bladder wall. The setup provides well-controlled thermal dose and the upscaling needed for performing large scale HIVEC experiments in rats.
Identifiants
pubmed: 35488115
doi: 10.1038/s41598-022-11016-y
pii: 10.1038/s41598-022-11016-y
pmc: PMC9054747
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7017Informations de copyright
© 2022. The Author(s).
Références
Cancer Res. 1986 Dec;46(12 Pt 1):6242-5
pubmed: 3779644
J Urol. 2010 Sep;184(3):990-4; quiz 1235
pubmed: 20643429
Cancer Lett. 1997 Oct 28;119(1):47-52
pubmed: 18372521
Int J Hyperthermia. 2020;37(1):854-860
pubmed: 32664768
Cell Tissue Res. 1990 Oct;262(1):67-79
pubmed: 2257617
Int J Hyperthermia. 2017 Nov;33(7):733-740
pubmed: 28540814
Cancers (Basel). 2021 Mar 12;13(6):
pubmed: 33808973
World J Urol. 2011 Feb;29(1):73-7
pubmed: 21132553
J Urol. 2005 Apr;173(4):1375-80
pubmed: 15758808
Lasers Med Sci. 2013 Jul;28(4):1143-50
pubmed: 23053245
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
Mol Cell. 2010 Oct 22;40(2):253-66
pubmed: 20965420
Front Oncol. 2020 Feb 05;10:29
pubmed: 32117709
Molecules. 2020 Jul 17;25(14):
pubmed: 32709143
BMC Urol. 2019 Dec 3;19(1):126
pubmed: 31795980
Acta Cir Bras. 2011;26 Suppl 2:106-10
pubmed: 22030824
Int J Hyperthermia. 2016 Jun;32(4):407-16
pubmed: 26939993
Int J Hyperthermia. 2003 Jan-Feb;19(1):45-57
pubmed: 12519711
Cancers (Basel). 2021 Jan 20;13(3):
pubmed: 33498535
Int J Hyperthermia. 2019;36(1):1147-1159
pubmed: 31752562
Bladder Cancer. 2017 Jul 27;3(3):201-210
pubmed: 28824948
Surg Oncol. 2016 Sep;25(3):281-97
pubmed: 27566035
Bladder Cancer. 2018 Oct 29;4(4):395-401
pubmed: 30417050
Cancers (Basel). 2021 Sep 15;13(18):
pubmed: 34572855
World J Urol. 2009 Jun;27(3):313-7
pubmed: 19219611
J Inorg Biochem. 2008 Apr;102(4):629-35
pubmed: 18037490
Int J Hyperthermia. 2014 May;30(3):166-70
pubmed: 24697672
Int J Nanomedicine. 2017 Apr 06;12:2793-2811
pubmed: 28435259
Radiat Oncol. 2015 Aug 07;10:165
pubmed: 26245485
Int J Hyperthermia. 2018;35(1):441-449
pubmed: 30303415
Int Urol Nephrol. 2016 Jan;48(1):79-84
pubmed: 26498630
Eur Urol. 2021 Apr;79(4):480-488
pubmed: 33419683
Int J Hyperthermia. 2016 Jun;32(4):434-45
pubmed: 27098923
Int J Hyperthermia. 2013 Dec;29(8):835-44
pubmed: 24050253
Mol Imaging. 2006 Jul;5(3):175-9
pubmed: 16954032
J Urol. 2015 Nov;194(5):1202-8
pubmed: 26143111
Cancer Chemother Pharmacol. 2014 Mar;73(3):503-9
pubmed: 24442034
Cancer Res. 1983 Jul;43(7):3231-5
pubmed: 6406056
Int J Nanomedicine. 2021 Jan 07;16:75-88
pubmed: 33447028
Eur Urol. 2016 Jun;69(6):1046-52
pubmed: 26803476
Adv Drug Deliv Rev. 2020;163-164:145-156
pubmed: 32247801
Proc SPIE Int Soc Opt Eng. 2013 Feb 26;8584:1656985
pubmed: 23837123
Eur Urol Oncol. 2019 May;2(3):239-247
pubmed: 31200837
Int J Hyperthermia. 2012;28(5):456-65
pubmed: 22690856
J Urol. 1990 Aug;144(2 Pt 1):370-4
pubmed: 2374209
Eur Urol. 2004 Nov;46(5):670-4
pubmed: 15474281
Int J Exp Pathol. 2003 Jun;84(3):145-52
pubmed: 12974944
Cells. 2020 Jul 25;9(8):
pubmed: 32722384