Current Status and Challenges of Aptamers Screening and Optimization.

Aptamers, Nucleotide / chemistry SELEX Aptamer Technique / methods
Aptamer SELEX antibodies immunogenicity optimization truncation

Journal

Combinatorial chemistry & high throughput screening
ISSN: 1875-5402
Titre abrégé: Comb Chem High Throughput Screen
Pays: United Arab Emirates
ID NLM: 9810948

Informations de publication

Date de publication:
2023
Historique:
received: 25 11 2021
revised: 14 02 2022
accepted: 21 02 2022
pubmed: 2 5 2022
medline: 28 3 2023
entrez: 1 5 2022
Statut: ppublish

Résumé

Aptamers, consisting of single-stranded DNA or RNA, have secondary and tertiary structures which could bind specifically to target molecules. They are characterized by strong specificity, high affinity, low molecular weight, and low immunogenicity; therefore, the current research focuses on their potential as a targeted drug carrier, a diagnostic probe for diseases, or as a direct therapeutic drug. In this review, how to improve the success rate of adaptor screening and the optimization after screening is described. For aptamer screening, an efficient selection strategy is needed. In this article, by analyzing key aspects of SELEX such as initial library design, screening procedures, truncation and modification after screening, a comprehensive analysis of each step that might meet obstacles in SELEX is provided. Aptamers, which possess the specificity and affinity with the target, can serve as targeted drug carriers or biosensors for diagnosing a disease. If the problems in the screening process in cell-SELEX technology, truncation, and modification after screening are solved, it will have a broader range of applications.

Sections du résumé

BACKGROUND
Aptamers, consisting of single-stranded DNA or RNA, have secondary and tertiary structures which could bind specifically to target molecules. They are characterized by strong specificity, high affinity, low molecular weight, and low immunogenicity; therefore, the current research focuses on their potential as a targeted drug carrier, a diagnostic probe for diseases, or as a direct therapeutic drug.
OBJECTIVE
In this review, how to improve the success rate of adaptor screening and the optimization after screening is described.
RESULTS
For aptamer screening, an efficient selection strategy is needed. In this article, by analyzing key aspects of SELEX such as initial library design, screening procedures, truncation and modification after screening, a comprehensive analysis of each step that might meet obstacles in SELEX is provided.
CONCLUSION
Aptamers, which possess the specificity and affinity with the target, can serve as targeted drug carriers or biosensors for diagnosing a disease. If the problems in the screening process in cell-SELEX technology, truncation, and modification after screening are solved, it will have a broader range of applications.

Identifiants

DOI: 10.2174/1386207325666220501170846 PMID: 35490432
pubmed: 35490432
pii: CCHTS-EPUB-123128
doi: 10.2174/1386207325666220501170846
doi:

Substances chimiques

Aptamers, Nucleotide 0

Types de publication

Review Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1067-1082

Informations de copyright

Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

Auteurs

Yong Tan (Y)

Medical College, China Three Gorges University, Yichang, Hubei, China.
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, Hubei, China.
Institute of Organ Fibrosis and Targeted Drug Delivery, China Three Gorges University, Yichang, Hubei, China.

Lan Ma (L)

Medical College, China Three Gorges University, Yichang, Hubei, China.
Institute of Organ Fibrosis and Targeted Drug Delivery, China Three Gorges University, Yichang, Hubei, China.

Xue Yang (X)

Medical College, China Three Gorges University, Yichang, Hubei, China.
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, Hubei, China.
Institute of Organ Fibrosis and Targeted Drug Delivery, China Three Gorges University, Yichang, Hubei, China.

Qi-Ni Cheng (QN)

Medical College, China Three Gorges University, Yichang, Hubei, China.
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, Hubei, China.
Institute of Organ Fibrosis and Targeted Drug Delivery, China Three Gorges University, Yichang, Hubei, China.

Jiang-Feng Wu (JF)

Medical College, China Three Gorges University, Yichang, Hubei, China.
Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, Hubei, China.
Institute of Organ Fibrosis and Targeted Drug Delivery, China Three Gorges University, Yichang, Hubei, China.
Department of Organ Fibrosis and Targeted Drug Delivery, the First People's Hospital of Yichang, Yichang, Hubei, Hubei, China.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › Current Status and Challenges of Aptamers...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de chimie combinatoire › Technique SELEX › Current Status and Challenges of Aptamers...