Analysis of senescence in gingival tissues and gingival fibroblast cultures.
aging
cytokines
hydrogen peroxide
inflammation
Journal
Clinical and experimental dental research
ISSN: 2057-4347
Titre abrégé: Clin Exp Dent Res
Pays: United States
ID NLM: 101692332
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
30
03
2022
received:
01
02
2022
accepted:
03
04
2022
pubmed:
3
5
2022
medline:
19
8
2022
entrez:
2
5
2022
Statut:
ppublish
Résumé
To determine senescence-associated changes in the gingival tissues of aged mice and gingival fibroblast cultures. The production of senescence-associated β-galactosidase (SA-β-gal) and mRNA expression of p16, p21, interleukin (IL)-1β, and tumor necrosis factor α (TNF-α) were evaluated in gingival tissues, gingival fibroblasts of 10- and 20-month-old C57BL/6NCrl mice, and multiple-passaged and hydrogen peroxide-stimulated human gingival fibroblasts (HGFs). Changes in molecular expression in HGF cultures due to senescent cell elimination by the senolytic drug ABT-263 (Navitoclax) were analyzed. Compared to 10-week-old mice, the 20-month-old mice had higher numbers of M1 macrophages. The proportion of cells expressing SA-β-gal were also higher in 20- month-old mice than in 10-week-old-mice. Gingival fibroblasts in 20-month-old mice expressed less collagen 1a1, collagen 4a1, and collagen 4a2 mRNA than those in 10-week-old mice. Compared to control cells, H2O2 treated HGF cells expressed higher levels of SA-β-gal and p16, p21, IL-1β, and TNF-α. Furthermore, ABT-263 suppressed HGF cell expression of cytokines after senescence induction. Senescence-associated changes were observed in the gingival tissues of aged mice and HGF cultures. In addition, the potential of senolytic drugs to modify aging-related changes in the gingiva was shown.
Identifiants
pubmed: 35491709
doi: 10.1002/cre2.581
pmc: PMC9382052
doi:
Substances chimiques
RNA, Messenger
0
Senotherapeutics
0
Tumor Necrosis Factor-alpha
0
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
939-949Informations de copyright
© 2022 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
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