Deconvolution of the hematopoietic stem cell microenvironment reveals a high degree of specialization and conservation.
Biological sciences
Omics
Stem cells research
Transcriptomics
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
20 May 2022
20 May 2022
Historique:
received:
17
11
2021
revised:
14
02
2022
accepted:
05
04
2022
entrez:
2
5
2022
pubmed:
3
5
2022
medline:
3
5
2022
Statut:
epublish
Résumé
Understanding the regulation of normal and malignant human hematopoiesis requires comprehensive cell atlas of the hematopoietic stem cell (HSC) regulatory microenvironment. Here, we develop a tailored bioinformatic pipeline to integrate public and proprietary single-cell RNA sequencing (scRNA-seq) datasets. As a result, we robustly identify for the first time 14 intermediate cell states and 11 stages of differentiation in the endothelial and mesenchymal BM compartments, respectively. Our data provide the most comprehensive description to date of the murine HSC-regulatory microenvironment and suggest a higher level of specialization of the cellular circuits than previously anticipated. Furthermore, this deep characterization allows inferring conserved features in human, suggesting that the layers of microenvironmental regulation of hematopoiesis may also be shared between species. Our resource and methodology is a stepping-stone toward a comprehensive cell atlas of the BM microenvironment.
Identifiants
pubmed: 35494238
doi: 10.1016/j.isci.2022.104225
pii: S2589-0042(22)00495-3
pmc: PMC9046238
doi:
Types de publication
Journal Article
Langues
eng
Pagination
104225Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Nat Immunol. 2019 Feb;20(2):163-172
pubmed: 30643263
Front Cell Dev Biol. 2020 Jul 08;8:561
pubmed: 32733882
Genome Biol. 2019 Dec 23;20(1):296
pubmed: 31870423
PLoS One. 2012;7(2):e32177
pubmed: 22359666
Gigascience. 2019 Oct 1;8(10):
pubmed: 31574155
Cell Mol Immunol. 2012 Jan;9(1):11-9
pubmed: 22020068
Eur J Pharmacol. 2009 May 21;610(1-3):61-7
pubmed: 19374835
J Transl Med. 2014 Nov 28;12 Suppl 2:S4
pubmed: 25471042
PLoS One. 2017 Apr 17;12(4):e0175632
pubmed: 28414795
J Am Heart Assoc. 2013 Dec 05;2(6):e000571
pubmed: 24308939
J Tissue Eng. 2023 May 16;14:20417314231169375
pubmed: 37216034
Cytometry A. 2022 Mar;101(3):237-253
pubmed: 33840138
Cell. 2020 Feb 20;180(4):764-779.e20
pubmed: 32059779
Cell. 2015 May 21;161(5):1202-1214
pubmed: 26000488
Microvasc Res. 2016 May;105:103-8
pubmed: 26860974
Thromb Haemost. 2009 Jun;101(6):1044-50
pubmed: 19492146
Front Cell Dev Biol. 2021 Apr 12;9:653308
pubmed: 33912565
Arch Biochem Biophys. 2019 Dec 15;678:108182
pubmed: 31704097
Am J Hematol. 1991 Dec;38(4):293-303
pubmed: 1746538
Exp Hematol. 2002 Jul;30(7):783-91
pubmed: 12135677
Nature. 2019 May;569(7755):222-228
pubmed: 30971824
Proc Natl Acad Sci U S A. 2021 Apr 13;118(15):
pubmed: 33827925
Nitric Oxide. 2015 Feb 15;45:20-6
pubmed: 25638487
Nat Biotechnol. 2021 Oct;39(10):1202-1215
pubmed: 33941931
Hepatology. 2009 Sep;50(3):920-8
pubmed: 19585615
Stem Cells Dev. 2016 Nov 1;25(21):1652-1658
pubmed: 27527928
Elife. 2020 Apr 14;9:
pubmed: 32286228
Matrix Biol. 2004 Nov;23(7):445-55
pubmed: 15579311
Sci Rep. 2017 Aug 31;7(1):10107
pubmed: 28860501
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13641-6
pubmed: 21808000
Bone. 2014 Dec;69:139-47
pubmed: 25260930
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
Commun Biol. 2020 Dec 4;3(1):736
pubmed: 33277618
Stem Cell Res Ther. 2020 Jan 7;11(1):16
pubmed: 31910882
Mol Cell Biochem. 2012 Mar;362(1-2):71-85
pubmed: 22038628
Biometrics. 1947 Sep;3(3):119-22
pubmed: 18903631
Nature. 2018 Jan 24;553(7689):418-426
pubmed: 29364285
Cell. 2021 Jun 24;184(13):3573-3587.e29
pubmed: 34062119
Blood. 2005 Apr 1;105(7):2852-61
pubmed: 15591117
Nature. 2021 Mar;591(7850):438-444
pubmed: 33627868
Nat Cell Biol. 2017 Apr;19(4):271-281
pubmed: 28319093
Cell Stem Cell. 2019 Oct 3;25(4):570-583.e7
pubmed: 31279774
Dev Cell. 2006 Dec;11(6):845-57
pubmed: 17141159
Int J Mol Sci. 2017 Oct 27;18(11):
pubmed: 29077057
Blood. 2020 Oct 15;136(16):1871-1883
pubmed: 32842144
Int Immunol. 2020 May 8;32(5):295-305
pubmed: 31996897
Cell Rep. 2018 Jan 16;22(3):666-678
pubmed: 29346765
BMC Syst Biol. 2009 Jun 04;3:56
pubmed: 19497118
Circ Res. 2005 Nov 25;97(11):1093-107
pubmed: 16306453
Science. 2020 Feb 14;367(6479):
pubmed: 31974159
Nature. 2010 Apr 8;464(7290):852-7
pubmed: 20305640
Nat Neurosci. 2016 Feb;19(2):335-46
pubmed: 26727548
Cell. 2019 Jun 13;177(7):1915-1932.e16
pubmed: 31130381
BMC Cardiovasc Disord. 2015 Oct 19;15:130
pubmed: 26481314
Nat Commun. 2020 Jan 16;11(1):332
pubmed: 31949165
Vitam Horm. 1985;42:65-108
pubmed: 3006348
Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18587-91
pubmed: 19843692
Cell Stem Cell. 2016 Nov 3;19(5):613-627
pubmed: 27666011
Int J Biol Sci. 2021 Oct 11;17(15):4192-4206
pubmed: 34803492
Front Cell Dev Biol. 2021 Mar 12;9:622519
pubmed: 33777933
Front Biosci. 2007 May 01;12:3068-92
pubmed: 17485283
Sci Rep. 2019 Mar 26;9(1):5233
pubmed: 30914743
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Nature. 2016 Apr 21;532(7599):323-8
pubmed: 27074509
Nat Cell Biol. 2018 Jul;20(7):836-846
pubmed: 29915358
Pediatr Res. 2013 Apr;73(4 Pt 2):502-7
pubmed: 23314297
Cell Stem Cell. 2009 Mar 6;4(3):263-74
pubmed: 19265665
J Biol Chem. 2007 Mar 16;282(11):8393-403
pubmed: 17194709
Physiol Rev. 2001 Oct;81(4):1415-59
pubmed: 11581493
Nature. 2018 Jan 11;553(7687):212-216
pubmed: 29323290
Nat Immunol. 2018 Jan;19(1):85-97
pubmed: 29167569
Nature. 2016 Jan 21;529(7586):316-25
pubmed: 26791722
Elife. 2016 Dec 13;5:
pubmed: 27976999
Sci Rep. 2021 Aug 4;11(1):15811
pubmed: 34349154
Exp Hematol. 2016 Jul;44(7):540-60
pubmed: 27179622
Cell Stem Cell. 2021 Apr 1;28(4):637-652.e8
pubmed: 33301706
Br J Haematol. 2021 May;193(3):659-668
pubmed: 33837967
Blood. 2016 Aug 25;128(8):e20-31
pubmed: 27365425
Cell Stem Cell. 2019 Dec 5;25(6):768-783.e6
pubmed: 31761723
Nat Cell Biol. 2020 Jan;22(1):38-48
pubmed: 31871321
J Cell Sci. 2012 Apr 1;125(Pt 7):1693-705
pubmed: 22328518
Cell Rep. 2019 Jul 9;28(2):302-311.e5
pubmed: 31291568
BMC Cell Biol. 2014 Dec 20;15:46
pubmed: 25526965