Protocol to assess human glioma propagating cell migration on linear micropatterns mimicking brain invasion tracks.

Biophysics Biotechnology and bioengineering Cancer Cell Biology Cell culture Cell-based Assays Microscopy

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 06 2022
Historique:
entrez: 2 5 2022
pubmed: 3 5 2022
medline: 4 5 2022
Statut: epublish

Résumé

Glioblastoma (GBM) cells invade the brain by following linear structures like blood vessel walls and white matter tracts by using specific motility modes. In this protocol, we describe two micropatterning techniques allowing recapitulation of these linear tracks

Identifiants

pubmed: 35496779
doi: 10.1016/j.xpro.2022.101331
pii: S2666-1667(22)00211-8
pmc: PMC9043773
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101331

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

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Auteurs

Michele Crestani (M)

IFOM - the Firc Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy.

Tania Dini (T)

IFOM - the Firc Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy.

Nils C Gauthier (NC)

IFOM - the Firc Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy.

Pascale Monzo (P)

IFOM - the Firc Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy.

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Classifications MeSH