Performance of the new biological small- and wide-angle X-ray scattering beamline 13A at the Taiwan Photon Source.
SAXS–WAXS
SWAXS
biomolecular solution scattering
integrated UV–Vis absorption and refractometry
online size exclusion chromatography
small-angle X-ray scattering
wide-angle X-ray scattering
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Apr 2022
01 Apr 2022
Historique:
received:
06
12
2021
accepted:
18
02
2022
entrez:
2
5
2022
pubmed:
3
5
2022
medline:
3
5
2022
Statut:
epublish
Résumé
Recent developments in the instrumentation and data analysis of synchrotron small-angle X-ray scattering (SAXS) on biomolecules in solution have made biological SAXS (BioSAXS) a mature and popular tool in structural biology. This article reports on an advanced endstation developed at beamline 13A of the 3.0 GeV Taiwan Photon Source for biological small- and wide-angle X-ray scattering (SAXS-WAXS or SWAXS). The endstation features an in-vacuum SWAXS detection system comprising two mobile area detectors (Eiger X 9M/1M) and an online size-exclusion chromatography system incorporating several optical probes including a UV-Vis absorption spectrometer and refractometer. The instrumentation and automation allow simultaneous SAXS-WAXS data collection and data reduction for high-throughput biomolecular conformation and composition determinations. The performance of the endstation is illustrated with the SWAXS data collected for several model proteins in solution, covering a scattering vector magnitude
Identifiants
pubmed: 35497659
doi: 10.1107/S1600576722001923
pii: S1600576722001923
pmc: PMC8985603
doi:
Types de publication
Journal Article
Langues
eng
Pagination
340-352Informations de copyright
© O. Shih et al. 2022.
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