Association of pleomorphic adenoma gene 1 with body weight and measurement of Bali cattle (
Bali cattle
Pleomorphic adenoma gene 1
association study
body length
growth trait
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
08
11
2021
accepted:
22
02
2022
entrez:
2
5
2022
pubmed:
3
5
2022
medline:
3
5
2022
Statut:
ppublish
Résumé
Pleomorphic adenoma gene 1 ( A total of 87 Bali cattle, consisting of 48 bulls and 39 heifers at the Breeding Center for Bali Cattle, were used as the population in this study. Cattle were 2 years old and kept semi-intensively in the pasture. Phenotype data consisting of body weight, withers height, body length, chest girth, waist height, and chest depth were measured. Birth weight data were obtained from birth records, and weight gain, adjusted weaning weight, and yearling weight were calculated using formulas. Blood samples were taken from the jugular vein as much as 5 mL, and genomic DNA was isolated using the salting-out method. Polymerase chain reaction (PCR) was performed to amplify three target polymorphisms, namely, g.48308 C>T, g.32212 (19 bp indel), and g.45233 T>C. The presence of a 19 bp indel was determined by direct observation of the PCR product on a 2% agarose gel. Two other polymorphisms were detected by PCR-restriction fragment length polymorphism using the restriction endonuclease enzymes The results showed that two of the target polymorphisms in The g.48308 C>T SNP in
Sections du résumé
Background and Aim
UNASSIGNED
Pleomorphic adenoma gene 1 (
Materials and Methods
UNASSIGNED
A total of 87 Bali cattle, consisting of 48 bulls and 39 heifers at the Breeding Center for Bali Cattle, were used as the population in this study. Cattle were 2 years old and kept semi-intensively in the pasture. Phenotype data consisting of body weight, withers height, body length, chest girth, waist height, and chest depth were measured. Birth weight data were obtained from birth records, and weight gain, adjusted weaning weight, and yearling weight were calculated using formulas. Blood samples were taken from the jugular vein as much as 5 mL, and genomic DNA was isolated using the salting-out method. Polymerase chain reaction (PCR) was performed to amplify three target polymorphisms, namely, g.48308 C>T, g.32212 (19 bp indel), and g.45233 T>C. The presence of a 19 bp indel was determined by direct observation of the PCR product on a 2% agarose gel. Two other polymorphisms were detected by PCR-restriction fragment length polymorphism using the restriction endonuclease enzymes
Results
UNASSIGNED
The results showed that two of the target polymorphisms in
Conclusion
UNASSIGNED
The g.48308 C>T SNP in
Identifiants
pubmed: 35497968
doi: 10.14202/vetworld.2022.782-788
pii: Vetworld-15-782
pmc: PMC9047134
doi:
Types de publication
Journal Article
Langues
eng
Pagination
782-788Informations de copyright
Copyright: © Cahyadi, et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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