LED Zappelin': An open source LED controller for arbitrary spectrum visual stimulation and optogenetics during 2-photon imaging.
2 Photon
LED
Light stimulation
Open hardware
Optogenetics
Visual neuroscience
Journal
HardwareX
ISSN: 2468-0672
Titre abrégé: HardwareX
Pays: England
ID NLM: 101710262
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
11
03
2020
revised:
16
07
2020
accepted:
16
07
2020
entrez:
2
5
2022
pubmed:
22
7
2020
medline:
22
7
2020
Statut:
epublish
Résumé
Two-photon (2P) microscopy is a cornerstone technique in neuroscience research. However, combining 2P imaging with spectrally arbitrary light stimulation can be challenging due to crosstalk between stimulation light and fluorescence detection. To overcome this limitation, we present a simple and low-cost electronic solution based on an ESP32 microcontroller and a TLC5947 LED driver to rapidly time-interleave stimulation and detection epochs during scans. Implemented for less than $100, our design can independently drive up to 24 arbitrary spectrum LEDs to meet user requirements. We demonstrate the utility of our stimulator for colour vision experiments on the
Identifiants
pubmed: 35498254
doi: 10.1016/j.ohx.2020.e00127
pii: S2468-0672(20)30036-5
pmc: PMC9041195
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00127Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
AMC has a consultancy company providing services for Open Science: Chagas Science Consultancy, registered in the UK 12,299,826The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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