Real-Time Fast Amyloid Seeding and Translocation of α-Synuclein with a Nanopipette.
Journal
ACS central science
ISSN: 2374-7943
Titre abrégé: ACS Cent Sci
Pays: United States
ID NLM: 101660035
Informations de publication
Date de publication:
27 Apr 2022
27 Apr 2022
Historique:
received:
15
11
2021
entrez:
4
5
2022
pubmed:
5
5
2022
medline:
5
5
2022
Statut:
ppublish
Résumé
The detection to α-synuclein (αS) assemblies as a biomarker of synucleinopathies is an important challenge for further development of an early diagnosis tool. Here, we present proof of concept real-time fast amyloid seeding and translocation (RT-FAST) based on a nanopipette that combines in one unique system a reaction vessel to accelerate the seed amplification and nanopore sensor for single-molecule αS assembly detection. RT-FAST allows the detection of the presence αS seeds WT and A53T variant in a given sample in only 90 min by adding a low quantity (35 μL at 100 nM) of recombinant αS for amplification. It also shows cross-seeding aggregation by adding mixing seeds A53T with WT monomers. Finally, we establish the dependence between the capture rate of aggregates by the nanopore sensor and the initial seed concentration from 200 pM to 2 pM, which promises further development toward a quantitative analysis of the initial seed concentration.
Identifiants
pubmed: 35505874
doi: 10.1021/acscentsci.1c01404
pmc: PMC9052795
doi:
Types de publication
Journal Article
Langues
eng
Pagination
441-448Informations de copyright
© 2022 The Authors. Published by American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
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