Integrated Analytical System for Clinical Single-Cell Analysis.
biopsy
cancer
deep profiling
diagnostic
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
revised:
31
03
2022
received:
21
01
2022
pubmed:
5
5
2022
medline:
19
7
2022
entrez:
4
5
2022
Statut:
ppublish
Résumé
High-dimensional analyses of cancers can potentially be used to better define cancer subtypes, analyze the complex tumor microenvironment, and perform cancer cell pathway analyses for drug trials. Unfortunately, integrated systems that allow such analyses in serial fine needle aspirates within a day or at point-of-care currently do not exist. To achieve this, an integrated immunofluorescence single-cell analyzer (i2SCAN) for deep profiling of directly harvested cells is developed. By combining a novel cellular imaging system, highly cyclable bioorthogonal FAST antibody panels, and integrated computational analysis, it is shown that same-day analysis is possible in thousands of harvested cells. It is demonstrated that the i2SCAN approach allows comprehensive analysis of breast cancer samples obtained by fine needle aspiration or core tissues. The method is a rapid, robust, and low-cost solution to high-dimensional analysis of scant clinical specimens.
Identifiants
pubmed: 35508767
doi: 10.1002/advs.202200415
pmc: PMC9284190
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200415Subventions
Organisme : Part of this work was funded by
ID : DOD-W81XWH1910194 (HL)
Organisme : Part of this work was funded by
ID : U01CA233360 (HL)
Organisme : Part of this work was funded by
ID : UH3CA202637 (RW)
Organisme : Part of this work was funded by
ID : R21CA217662 (HI)
Organisme : NCI NIH HHS
ID : T32 CA079443
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA229777
Pays : United States
Organisme : Part of this work was funded by
ID : R01GM138779 (HI)
Organisme : Part of this work was funded by
ID : MGH Scholar Fund (HL)
Organisme : NIGMS NIH HHS
ID : R01 GM138778
Pays : United States
Organisme : NCI NIH HHS
ID : UH3 CA202637
Pays : United States
Organisme : Part of this work was funded by
ID : R01CA257623 (RW)
Organisme : NCI NIH HHS
ID : R21 CA217662
Pays : United States
Organisme : Part of this work was funded by
ID : T32CA079443 (HMP)
Organisme : NCI NIH HHS
ID : R01 CA257623
Pays : United States
Organisme : MGH Scholar Fund
ID : T32CA079443
Organisme : Part of this work was funded by
ID : R01CA229777 (HL)
Organisme : NCI NIH HHS
ID : U01 CA233360
Pays : United States
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Cancer Commun (Lond). 2020 Apr;40(4):135-153
pubmed: 32301585
Cell Syst. 2020 Sep 23;11(3):272-285.e9
pubmed: 32898474
Phys Med. 2009 Sep;25(3):128-32
pubmed: 19419893
Nat Commun. 2018 Oct 31;9(1):4550
pubmed: 30382095
Sci Transl Med. 2020 Aug 5;12(555):
pubmed: 32759277
Nat Biotechnol. 2005 Mar;23(3):313-20
pubmed: 15765087
Clin Cancer Res. 2021 Sep 1;27(17):4781-4793
pubmed: 34233961
Nat Biotechnol. 2022 Apr;40(4):555-565
pubmed: 34795433
Nat Methods. 2021 Jan;18(1):100-106
pubmed: 33318659
Sci Transl Med. 2014 Jan 15;6(219):219ra9
pubmed: 24431113
Endocrine. 2015 Aug;49(3):745-51
pubmed: 25649761
Nat Methods. 2021 Jan;18(1):43-45
pubmed: 33398191
IEEE Trans Med Imaging. 2020 Jan;39(1):62-74
pubmed: 31150339
J Am Chem Soc. 2020 Nov 11;142(45):19132-19141
pubmed: 33119297
ACS Appl Mater Interfaces. 2021 May 19;13(19):22124-22130
pubmed: 33870697
Nat Med. 2003 Jan;9(1):123-8
pubmed: 12514725
Cancer Cytopathol. 2020 Nov;128(11):782-791
pubmed: 32841527
Nat Biomed Eng. 2022 May;6(5):515-526
pubmed: 34750536
ACS Nano. 2018 Sep 25;12(9):9081-9090
pubmed: 30113824
Nature. 2020 Feb;578(7796):615-620
pubmed: 31959985
Science. 2021 Jun 11;372(6547):
pubmed: 34112666
Pediatr Radiol. 2016 Mar;46(3):372-82
pubmed: 26589305
Oncologist. 2013;18(7):777-8
pubmed: 23882018
Cell. 2018 Aug 9;174(4):968-981.e15
pubmed: 30078711
Nat Commun. 2019 Dec 11;10(1):5662
pubmed: 31827096
Adv Sci (Weinh). 2022 Jul;9(20):e2200415
pubmed: 35508767
JCO Precis Oncol. 2017 Nov;1:1-9
pubmed: 35172508
J Am Chem Soc. 2018 Mar 14;140(10):3603-3612
pubmed: 29384666
J Ultrason. 2017 Dec;17(71):267-274
pubmed: 29375902
Eur J Immunol. 2019 Oct;49(10):1457-1973
pubmed: 31633216
Angew Chem Int Ed Engl. 2020 Apr 20;59(17):6839-6846
pubmed: 32004403