Untargeted Metabolomic Analysis of Nonvolatile and Volatile Glucosinolates in Brassicaceae.
Brassica vegetables
Glucosinolates
Isothiocyanates
Mass spectrometry
Untargeted metabolomic analysis
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
4
5
2022
pubmed:
5
5
2022
medline:
7
5
2022
Statut:
ppublish
Résumé
Glucosinolates (GLSs) are a group of plant secondary metabolites mainly found in Cruciferous plants. The main hydrolysis products of GLSs, isothiocyanates (ITCs), are the bioactive metabolites that have shown plant defense and human cancer prevention properties. Untargeted metabolomic analysis of plant metabolites can uncover the profiles of these bioactive phytochemicals in specific plants and discover potential human health promoting products. We have developed an integrated metabolomic analysis method for plant samples, with specific focus on nonvolatile GLSs and volatile ITCs. In this chapter, we describe in detail the protocols, including metabolite extraction, high resolution LC-MS and GC-MS analysis, and data processing. The method is readily applicable to untargeted analysis of other nonvolatile and volatile metabolites in plants.
Identifiants
pubmed: 35508842
doi: 10.1007/978-1-0716-2185-1_18
doi:
Substances chimiques
Glucosinolates
0
Isothiocyanates
0
Phytochemicals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
219-229Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Blažević I, Montaut S, Burčul F, Olsen CE, Burow M, Rollin P, Agerbirk N (2020) Glucosinolate structural diversity, identification, chemical synthesis and metabolism in plants. Phytochemistry 169:112100
doi: 10.1016/j.phytochem.2019.112100
Singh SV, Singh K (2012) Cancer chemoprevention with dietary isothiocyanates mature for clinical translational research. Carcinogenesis 33(10):1833–1842
doi: 10.1093/carcin/bgs216
Clavijo Mccormick A, Unsicker SB, Gershenzon J (2012) The specificity of herbivore-induced plant volatiles in attracting herbivore enemies: specificity in plant-enemy interactions. Trends Plant Sci 17(5):303–310
doi: 10.1016/j.tplants.2012.03.012
Sundaram MK, Preetha R, Haque S, Akhter N, Khan S, Ahmed S, Hussain A (2021) Dietary isothiocyanates inhibit cancer progression by modulation of epigenome. Semin Cancer Biol (online first). https://doi.org/10.1016/j.semcancer.2020.12.021
Agerbirk N, Olsen CE (2012) Glucosinolate structures in evolution. Phytochemistry 77:16–45
doi: 10.1016/j.phytochem.2012.02.005
Liu Y, Zhang H, Umashankar S, Liang X, Lee HW, Swarup S, Ong CN (2018) Characterization of plant volatiles reveals distinct metabolic profiles and pathways among 12 Brassicaceae vegetables. Metabolites 8(4):94. https://doi.org/10.3390/metabo8040094
doi: 10.3390/metabo8040094
pmcid: 6316591