Metagenomic Next-Generation Sequencing vs. Traditional Pathogen Detection in the Diagnosis of Infection After Allogeneic Hematopoietic Stem Cell Transplantation in Children.
allogeneic hematopoietic stem cell transplantation
central nervous system infections
metagenomic next-generation sequencing
pediatric
pulmonary infection
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2022
2022
Historique:
received:
02
02
2022
accepted:
21
03
2022
entrez:
5
5
2022
pubmed:
6
5
2022
medline:
6
5
2022
Statut:
epublish
Résumé
Infection is a severe complication of allo-HSCT in children, however, the accurate detection of the infection is hard. In this study, we traced the records of 101 pediatric recipients with allo-HSCT to investigate the pathogens of infection, and collected 54 bronchoalveolar lavage fluid, 32 blood, and 15 cerebrospinal fluid samples. In these samples, 87 was with post-transplant infection and 14 without infection. Using the metagenomic next-generation sequencing (mNGS) and traditional pathogen detection, we compared their sensitivity and specificity to detect pathogens of infection. Our results showed that mNGS was more sensitive (89.7%) than conventional pathogen detection (21.8%), with a difference of 67.9% (
Identifiants
pubmed: 35509305
doi: 10.3389/fmicb.2022.868160
pmc: PMC9058167
doi:
Types de publication
Journal Article
Langues
eng
Pagination
868160Informations de copyright
Copyright © 2022 Qu, Ding, Liu, Wang, Wang, Liu, Xia, Chen and Jiang.
Déclaration de conflit d'intérêts
HX was employed by Hugobiotech Co., Ltd. YC was employed by BGI PathoGenesis Pharmaceutical Technology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Infect Drug Resist. 2020 Feb 19;13:567-576
pubmed: 32110067
Genome Res. 2019 May;29(5):831-842
pubmed: 30992304
Biol Blood Marrow Transplant. 2015 Jun;21(6):1091-8
pubmed: 25708217
Bone Marrow Transplant. 2019 Aug;54(8):1354-1360
pubmed: 30696997
Clin Chest Med. 2017 Sep;38(3):479-491
pubmed: 28797490
Curr Opin Infect Dis. 2020 Apr;33(2):205-213
pubmed: 31990810
Am J Respir Crit Care Med. 2018 Feb 15;197(4):524-528
pubmed: 28686513
Science. 2006 Jun 2;312(5778):1355-9
pubmed: 16741115
Expert Rev Mol Diagn. 2016 Sep;16(9):1011-23
pubmed: 27453996
Clin Infect Dis. 2001 Jan;32(1):E1-8
pubmed: 11118397
J Thorac Dis. 2016 Mar;8(3):505-12
pubmed: 27076947
PLoS One. 2013 Oct 04;8(10):e77805
pubmed: 24124621
J Biotechnol. 2017 Feb 10;243:16-24
pubmed: 28042011
N Engl J Med. 2019 Jun 13;380(24):2327-2340
pubmed: 31189036
J Int Med Res. 2019 Oct;47(10):4878-4885
pubmed: 31436107
Clin Infect Dis. 2018 Nov 13;67(suppl_2):S231-S240
pubmed: 30423048
Ann Oncol. 2016 Jul;27(7):1207-25
pubmed: 27052648
Eur J Haematol. 2017 May;98(5):450-458
pubmed: 28129450
Methods Mol Biol. 2014;1096:183-201
pubmed: 24515370
Biol Blood Marrow Transplant. 2017 Jan;23(1):134-139
pubmed: 27794456
Bone Marrow Transplant. 2021 Aug;56(8):1978-1983
pubmed: 33824437
BMC Pulm Med. 2020 Oct 15;20(1):268
pubmed: 33059646
Hematol Oncol Stem Cell Ther. 2017 Mar;10(1):22-28
pubmed: 27664550
J Postgrad Med. 2008 Jul-Sep;54(3):203-5
pubmed: 18626168
J Infect Chemother. 2016 Aug;22(8):505-14
pubmed: 27344206
J Infect. 2019 Feb;78(2):158-169
pubmed: 30149030
Bone Marrow Transplant. 1999 Jun;23(11):1167-76
pubmed: 10382957