Histone Variant macroH2A1.1 Enhances Nonhomologous End Joining-dependent DNA Double-strand-break Repair and Reprogramming Efficiency of Human iPSCs.
DNA damage
cell reprogramming
induced pluripotent stem cells
macroH2A1.1
Journal
Stem cells (Dayton, Ohio)
ISSN: 1549-4918
Titre abrégé: Stem Cells
Pays: England
ID NLM: 9304532
Informations de publication
Date de publication:
03 03 2022
03 03 2022
Historique:
received:
04
06
2021
accepted:
02
09
2021
entrez:
5
5
2022
pubmed:
6
5
2022
medline:
10
5
2022
Statut:
ppublish
Résumé
DNA damage repair (DDR) is a safeguard for genome integrity maintenance. Increasing DDR efficiency could increase the yield of induced pluripotent stem cells (iPSC) upon reprogramming from somatic cells. The epigenetic mechanisms governing DDR during iPSC reprogramming are not completely understood. Our goal was to evaluate the splicing isoforms of histone variant macroH2A1, macroH2A1.1, and macroH2A1.2, as potential regulators of DDR during iPSC reprogramming. GFP-Trap one-step isolation of mtagGFP-macroH2A1.1 or mtagGFP-macroH2A1.2 fusion proteins from overexpressing human cell lines, followed by liquid chromatography-tandem mass spectrometry analysis, uncovered macroH2A1.1 exclusive interaction with Poly-ADP Ribose Polymerase 1 (PARP1) and X-ray cross-complementing protein 1 (XRCC1). MacroH2A1.1 overexpression in U2OS-GFP reporter cells enhanced specifically nonhomologous end joining (NHEJ) repair pathway, while macroH2A1.1 knock-out (KO) mice showed an impaired DDR capacity. The exclusive interaction of macroH2A1.1, but not macroH2A1.2, with PARP1/XRCC1, was confirmed in human umbilical vein endothelial cells (HUVEC) undergoing reprogramming into iPSC through episomal vectors. In HUVEC, macroH2A1.1 overexpression activated transcriptional programs that enhanced DDR and reprogramming. Consistently, macroH2A1.1 but not macroH2A1.2 overexpression improved iPSC reprogramming. We propose the macroH2A1 splicing isoform macroH2A1.1 as a promising epigenetic target to improve iPSC genome stability and therapeutic potential.
Identifiants
pubmed: 35511867
pii: 6511687
doi: 10.1093/stmcls/sxab004
pmc: PMC9199840
doi:
Substances chimiques
Histones
0
MACROH2A1 protein, human
0
X-ray Repair Cross Complementing Protein 1
0
XRCC1 protein, human
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
35-48Subventions
Organisme : Wellcome Trust
ID : 208375/Z/17/Z
Pays : United Kingdom
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Références
Genes (Basel). 2017 Dec 05;8(12):
pubmed: 29206173
Nat Commun. 2017 Oct 27;8(1):1158
pubmed: 29079819
PLoS One. 2017 Sep 21;12(9):e0185196
pubmed: 28934364
Cell Stem Cell. 2020 Oct 1;27(4):523-531
pubmed: 33007237
Stem Cell Res Ther. 2017 Mar 21;8(1):73
pubmed: 28327192
Cancer Lett. 2013 Aug 19;336(2):247-52
pubmed: 23531411
Cell Rep. 2014 Aug 21;8(4):1049-62
pubmed: 25131201
Mol Cell. 2018 Jan 4;69(1):36-47.e7
pubmed: 29249653
Cell Rep. 2013 Apr 25;3(4):1005-11
pubmed: 23545500
Methods Mol Biol. 2012;920:379-91
pubmed: 22941618
Cancer Res. 2016 Feb 1;76(3):594-606
pubmed: 26772755
Hum Mol Genet. 2001 May 1;10(10):1101-13
pubmed: 11331621
DNA Repair (Amst). 2018 Aug;68:34-40
pubmed: 29929045
Int J Mol Sci. 2017 Sep 13;18(9):
pubmed: 28902128
Stem Cells Dev. 2018 Jan 1;27(1):10-22
pubmed: 29117787
Hepatology. 2018 Feb;67(2):636-650
pubmed: 28913935
Cancers (Basel). 2019 May 15;11(5):
pubmed: 31096699
World J Stem Cells. 2019 Jul 26;11(7):421-430
pubmed: 31396369
Epigenetics Chromatin. 2016 Oct 25;9:45
pubmed: 27800025
Cell. 2006 Aug 25;126(4):663-76
pubmed: 16904174
OMICS. 2006 Spring;10(1):15-27
pubmed: 16584315
Ann N Y Acad Sci. 2010 Mar;1192:38-44
pubmed: 20392216
J Cell Biol. 2008 Oct 20;183(2):223-39
pubmed: 18936248
Cell Stem Cell. 2017 Apr 6;20(4):462-477.e6
pubmed: 28111200
Mol Cell Biol. 2019 Dec 11;40(1):
pubmed: 31636161
Curr Protoc Mol Biol. 2005 May;Chapter 28:Unit 28.1
pubmed: 18265366
Brain Res Bull. 2018 Jun;140:5-18
pubmed: 29605488
Nature. 2018 May;557(7707):619-620
pubmed: 29844563
Nat Commun. 2017 Nov 13;8(1):1470
pubmed: 29133916
Nat Rev Genet. 2016 Aug;17(8):487-500
pubmed: 27346641
Nat Struct Mol Biol. 2019 Mar;26(3):213-219
pubmed: 30833786
Stem Cells Int. 2015;2015:628368
pubmed: 25767512
Front Genet. 2018 Oct 09;9:417
pubmed: 30356649
Mol Cell. 2020 Sep 3;79(5):836-845.e7
pubmed: 32649884
Mol Cell Oncol. 2018 Apr 4;5(3):e1441629
pubmed: 30250894
PLoS One. 2013;8(1):e54458
pubmed: 23372727
Genesis. 2004 Mar;38(3):151-8
pubmed: 15048813
Nature. 2009 Oct 22;461(7267):1071-8
pubmed: 19847258
Cell Rep. 2020 Jun 2;31(9):107704
pubmed: 32492420
Mol Cell. 2018 Jul 19;71(2):319-331.e3
pubmed: 29983321
Sci Rep. 2018 Jan 16;8(1):841
pubmed: 29339820
J Biol Chem. 2004 Dec 31;279(53):55117-26
pubmed: 15498778
J Alzheimers Dis. 2017;59(2):615-631
pubmed: 28671110
Biology (Basel). 2021 Jul 13;10(7):
pubmed: 34356514
Nat Rev Mol Cell Biol. 2008 Apr;9(4):297-308
pubmed: 18285803
Aging (Albany NY). 2020 Aug 5;12(14):13979-13990
pubmed: 32634782
Nat Struct Mol Biol. 2009 Sep;16(9):923-9
pubmed: 19680243
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
Nat Commun. 2013;4:1565
pubmed: 23463008
Sci Rep. 2015 Nov 30;5:17259
pubmed: 26616161
Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):17141-6
pubmed: 21969577
Nat Biotechnol. 2019 Oct;37(10):1198-1208
pubmed: 31501559
Mol Cell Biol. 2011 Oct;31(20):4244-55
pubmed: 21844227
Sci Rep. 2021 Mar 1;11(1):4849
pubmed: 33649352
Oncotarget. 2016 May 31;7(22):32821-34
pubmed: 27092877
DNA Repair (Amst). 2019 Sep;81:102664
pubmed: 31324530
Cell Cycle. 2015;14(24):3851-63
pubmed: 26645646
J Cell Physiol. 2016 Jan;231(1):15-24
pubmed: 26033759
Antioxid Redox Signal. 2021 Feb 1;34(4):335-349
pubmed: 32567336
Nat Cell Biol. 2016 Dec 23;19(1):1-9
pubmed: 28008184
Cells. 2019 Sep 17;8(9):
pubmed: 31533340
Cell Prolif. 2019 Nov;52(6):e12674
pubmed: 31517418
Nucleic Acids Res. 2006;34(21):6170-82
pubmed: 17088286
Mutagenesis. 2020 Feb 13;35(1):1-3
pubmed: 32052057
FASEB J. 2019 Nov;33(11):11668-11681
pubmed: 31348687
Front Oncol. 2018 Oct 30;8:459
pubmed: 30425964
Nucleic Acids Res. 2015 Jan;43(Database issue):D1124-32
pubmed: 25361971
Epigenetics. 2016 Oct 2;11(10):709-720
pubmed: 27646854
Mutat Res. 2013 Jan-Mar;752(1):25-35
pubmed: 23010441
Stem Cell Reports. 2018 Aug 14;11(2):485-496
pubmed: 30033087
Stem Cells. 2017 Apr;35(4):952-966
pubmed: 28207177
Nat Struct Mol Biol. 2012 Nov;19(11):1139-46
pubmed: 23022728
Cells. 2019 Jan 07;8(1):
pubmed: 30621042
Genomics Proteomics Bioinformatics. 2016 Jun;14(3):131-139
pubmed: 27240471
Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2415-20
pubmed: 18272492
Nat Rev Mol Cell Biol. 2017 May;18(5):299-314
pubmed: 28144029
Nat Med. 2013 Aug;19(8):998-1004
pubmed: 23921754
PLoS One. 2012;7(3):e30541
pubmed: 22412831
Aging (Albany NY). 2014 Jan;6(1):35-47
pubmed: 24473773
Clin Epigenetics. 2019 Aug 22;11(1):121
pubmed: 31439048
Neurobiol Dis. 2021 Jun;153:105303
pubmed: 33631273
Front Microbiol. 2017 Jun 22;8:1157
pubmed: 28690599