Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
13
01
2022
accepted:
21
04
2022
entrez:
5
5
2022
pubmed:
6
5
2022
medline:
10
5
2022
Statut:
epublish
Résumé
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients taken from the tumor center and tumor margin. Two subtypes of NSCLC, adenocarcinoma (ADC) and squamous cell carcinoma (SCC) were compared. Data are available via ProteomeXchange with identifier PXD032736 and PXD032962 for ADC and SCC, respectively. For ADC proteins, 26 significant canonical pathways were identified, including Rho signaling pathways, a semaphorin neuronal repulsive signaling pathway, and epithelial adherens junction signaling. For SCC proteins, nine significant canonical pathways were identified, including hypoxia-inducible factor-1α signaling, thyroid hormone biosynthesis, and phagosome maturation. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, EMC transition, epithelial adherens junctions, and inflammatory responses. In conclusion, we identified several proteins that are important for the better characterization of tumor development and molecular specificity of both lung cancer subtypes. We also identified proteins that may be important as biomarkers and/or targets for anticancer therapy.
Identifiants
pubmed: 35512017
doi: 10.1371/journal.pone.0268073
pii: PONE-D-22-01172
pmc: PMC9071164
doi:
Substances chimiques
Biomarkers, Tumor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0268073Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Autophagy. 2018;14(11):1943-1958
pubmed: 30112966
Cell Oncol (Dordr). 2018 Oct;41(5):527-539
pubmed: 30088263
Cancers (Basel). 2019 Dec 21;12(1):
pubmed: 31877723
Sci Rep. 2019 May 17;9(1):7507
pubmed: 31101846
Int J Mol Sci. 2018 Dec 30;20(1):
pubmed: 30598022
Gene. 2020 Oct 30;759:144994
pubmed: 32721475
Adv Clin Chem. 2015;72:107-70
pubmed: 26471082
Front Oncol. 2021 Apr 16;11:676077
pubmed: 33937086
Adv Med Sci. 2017 Sep;62(2):405-413
pubmed: 28646744
J Proteome Res. 2011 Jan 7;10(1):269-76
pubmed: 21047053
Front Med (Lausanne). 2021 Sep 22;8:747333
pubmed: 34631760
J Cancer. 2019 Jan 1;10(1):51-60
pubmed: 30662525
Zhongguo Fei Ai Za Zhi. 2008 Jun 20;11(3):391-7
pubmed: 20731941
Transl Cancer Res. 2020 Jan;9(1):21-29
pubmed: 35117154
Int J Environ Res Public Health. 2021 Jan 14;18(2):
pubmed: 33466597
PLoS One. 2018 Jan 17;13(1):e0191377
pubmed: 29342219
BMC Pulm Med. 2021 Jul 14;21(1):234
pubmed: 34261474
Mol Carcinog. 2020 Jul;59(7):754-765
pubmed: 32363633
J Intern Med. 2014 Jul;276(1):41-51
pubmed: 24661605
Anticancer Drugs. 2017 Aug;28(7):757-770
pubmed: 28471809
J Vis Exp. 2013 Dec 30;(82):50948
pubmed: 24429481
Cancer Biomark. 2011-2012;10(1):3-12
pubmed: 22297547
Exp Hematol Oncol. 2015 Jun 12;4:16
pubmed: 26146591
Cell Syst. 2019 Aug 28;9(2):109-127
pubmed: 31465728
Dev Cell. 2019 May 6;49(3):302-303
pubmed: 31063745
Nucleic Acids Res. 2016 Dec 15;44(22):11033
pubmed: 27683222
PLoS One. 2017 Nov 7;12(11):e0187246
pubmed: 29112949
J Exp Med. 2019 Jun 3;216(6):1377-1395
pubmed: 31015297
Am J Cancer Res. 2012;2(1):93-103
pubmed: 22206048
Tumour Biol. 2016 Jan;37(1):553-9
pubmed: 26232327
J Cancer. 2015 Aug 20;6(10):990-5
pubmed: 26366212
Drug Resist Updat. 2016 Nov;29:1-12
pubmed: 27912840
Tumour Biol. 2016 Oct;37(10):14321-14328
pubmed: 27599983
Adv Respir Med. 2021;89(4):419-426
pubmed: 34494245
J Cancer Res Ther. 2015 Aug;11 Suppl 1:C104-6
pubmed: 26323904
J Steroid Biochem Mol Biol. 2021 Apr;208:105786
pubmed: 33189851
Oncogene. 2019 Jan;38(1):17-32
pubmed: 30072740
Physiol Rev. 2021 Jan 1;101(1):319-352
pubmed: 32584192
Cell Mol Biol Lett. 2020 Jan 16;25:1
pubmed: 31988639
Vopr Onkol. 1980;26(4):102-4
pubmed: 7376538
Oncotarget. 2017 Jan 17;8(3):4501-4519
pubmed: 27966459
Mol Cancer Res. 2012 Oct;10(10):1319-31
pubmed: 22912335
Eur J Cancer. 2021 Oct;156:35-45
pubmed: 34418665
Cancers (Basel). 2020 May 01;12(5):
pubmed: 32369931
J Proteomics. 2020 Jan 16;211:103557
pubmed: 31669361
J Cancer. 2018 Nov 24;9(24):4650-4658
pubmed: 30588249
Front Oncol. 2015 Apr 07;5:75
pubmed: 25905039
Mol Cancer Res. 2014 Jan;12(1):24-31
pubmed: 24398390
Int J Radiat Oncol Biol Phys. 2022 Feb 1;112(2):529-541
pubmed: 34506873
Respir Res. 2018 Mar 7;19(1):41
pubmed: 29514686
Proteomics Clin Appl. 2016 Nov;10(11):1073-1076
pubmed: 27377180
Oncotarget. 2018 Jan 25;9(10):9400-9414
pubmed: 29507698
Trends Mol Med. 2019 Apr;25(4):303-314
pubmed: 30824197
J Thorac Oncol. 2022 Mar;17(3):399-410
pubmed: 34740862
Cancer Lett. 2016 Jun 28;376(1):118-26
pubmed: 27012188
Respir Res. 2019 Mar 4;20(1):48
pubmed: 30832674
Front Oncol. 2020 Aug 28;10:1620
pubmed: 32984031
Am J Transl Res. 2019 Feb 15;11(2):612-623
pubmed: 30899366
J Int Med Res. 2013 Jun;41(3):573-83
pubmed: 23613495
Am J Physiol Lung Cell Mol Physiol. 2017 Oct 1;313(4):L664-L676
pubmed: 28619761
BMC Pulm Med. 2020 Aug 12;20(1):213
pubmed: 32787827
Cancers (Basel). 2020 Aug 02;12(8):
pubmed: 32748838
Semin Cancer Biol. 2020 Feb;60:107-120
pubmed: 31369816
J Proteomics. 2016 Apr 14;138:95-105
pubmed: 26898345
Ann Oncol. 1999;10 Suppl 5:S95-102
pubmed: 10582149
J Pharm Biomed Anal. 2014 Dec;101:40-9
pubmed: 25175018
Cancers (Basel). 2020 Aug 27;12(9):
pubmed: 32867043
PLoS One. 2019 Oct 17;14(10):e0223840
pubmed: 31622403
Clin Cancer Res. 2012 Oct 15;18(20):5585-94
pubmed: 23071360
Transl Cancer Res. 2019 Dec;8(8):2725-2735
pubmed: 35117030
Sci Transl Med. 2012 Mar 28;4(127):127ps10
pubmed: 22461637
Genomics. 2005 Dec;86(6):627-37
pubmed: 16269234
Oncol Rep. 2017 Dec;38(6):3659-3667
pubmed: 29039571
Thorac Cancer. 2013 Nov;4(4):422-432
pubmed: 28920217
J Proteome Res. 2007 Mar;6(3):1083-92
pubmed: 17284061
Reprod Fertil Dev. 2011;23(1):81-93
pubmed: 21366984
Expert Rev Mol Diagn. 2016;16(4):399-405
pubmed: 26694525
Breast Cancer Res. 2014 Mar 17;16(2):R26
pubmed: 24636070
EMBO Mol Med. 2013 Feb;5(2):309-25
pubmed: 23300147
Folia Histochem Cytobiol. 2012 Oct 08;50(3):368-74
pubmed: 23042266
Oncotarget. 2017 Nov 25;8(66):110273-110288
pubmed: 29299146
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):5479-84
pubmed: 25847991
Clin Cancer Res. 2020 Jan 1;26(1):274-281
pubmed: 31548347
Bioinformatics. 2014 Feb 15;30(4):523-30
pubmed: 24336805
Cancer Lett. 2009 Jan 8;273(1):1-14
pubmed: 18625544
Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):7004-9
pubmed: 21487000
Pathol Res Pract. 2012 Sep 15;208(9):534-40
pubmed: 22824147
Cancers (Basel). 2020 Jun 16;12(6):
pubmed: 32560247
J Enzyme Inhib Med Chem. 2020 Dec;35(1):1450-1461
pubmed: 32648529
Curr Oncol. 2022 Jan 21;29(2):479-489
pubmed: 35200543
Mediators Inflamm. 2017;2017:3827841
pubmed: 28490837
Int J Mol Med. 2018 Mar;41(3):1377-1384
pubmed: 29286069
Mol Cancer Ther. 2008 Nov;7(11):3470-9
pubmed: 19001432
JAMA Dermatol. 2016 May 1;152(5):533-40
pubmed: 26792250
Cancer Epidemiol Biomarkers Prev. 2009 Jun;18(6):1829-40
pubmed: 19505916
Pharmacol Ther. 2015 Jun;150:94-108
pubmed: 25595323
J Biol Regul Homeost Agents. 2021 Feb 26;35(2):537-546
pubmed: 33631924
Oncol Rep. 2018 Feb;39(2):721-730
pubmed: 29207182
J Endocrinol. 2016 Apr;229(1):R17-28
pubmed: 26795718
Oncogene. 2015 Sep 24;34(39):5025-36
pubmed: 25531318
Cancer Cell. 2019 May 13;35(5):721-737.e9
pubmed: 31056398
J Exp Clin Cancer Res. 2019 Apr 25;38(1):174
pubmed: 31023336
Oncol Rep. 2010 Aug;24(2):465-71
pubmed: 20596634
Cancer Metastasis Rev. 2007 Jun;26(2):319-31
pubmed: 17458507
Oncol Rep. 2010 Sep;24(3):637-45
pubmed: 20664968
PLoS One. 2013;8(2):e57650
pubmed: 23460890
Front Oncol. 2021 Oct 07;11:772811
pubmed: 34692554
BMC Cancer. 2013 Feb 01;13:44
pubmed: 23374247
Cancer Immunol Res. 2017 Sep;5(9):812-820
pubmed: 28811289
Tumour Biol. 2014 Sep;35(9):9371-80
pubmed: 24946721
Nat Commun. 2019 May 13;10(1):2130
pubmed: 31086178
Cancer Res. 2008 Oct 15;68(20):8295-303
pubmed: 18922901
Clin Transl Oncol. 2013 Sep;15(9):671-82
pubmed: 23606351
Nat Chem Biol. 2016 Jul;12(7):511-5
pubmed: 27182663
Oncol Lett. 2018 Nov;16(5):5753-5760
pubmed: 30344729
J Cell Physiol. 2019 Dec;234(12):23798-23806
pubmed: 31180588
Front Oncol. 2022 Jan 13;11:793121
pubmed: 35096591
PLoS One. 2013 Sep 05;8(9):e73710
pubmed: 24040035
Curr Pharm Des. 2021;27(46):4663-4670
pubmed: 34455968
Cancer. 2001 Mar 15;91(6):1129-41
pubmed: 11267958
Free Radic Biol Med. 2015 Sep;86:1-15
pubmed: 25881549
Proteomics. 2014 Mar;14(6):795-803
pubmed: 24453208
J Pathol Clin Res. 2022 Jan;8(1):33-47
pubmed: 34647699
Cancer Res. 2000 Jun 1;60(11):3088-95
pubmed: 10850461
Biomed Res. 2012;33(5):309-17
pubmed: 23124251
Mol Cell Proteomics. 2012 Feb;11(2):M111.008821
pubmed: 22064513
Cells. 2022 Jan 27;11(3):
pubmed: 35159257
Science. 1993 Mar 5;259(5100):1409-10
pubmed: 8451637
Carcinogenesis. 2016 Mar;37(3):280-289
pubmed: 26905588
Clin Proteomics. 2022 Jan 7;19(1):2
pubmed: 34996345
Biochem Biophys Res Commun. 2018 Apr 15;498(4):932-939
pubmed: 29545175
Mol Med Rep. 2015 Nov;12(5):6399-404
pubmed: 26324337
Cancers (Basel). 2021 Aug 06;13(16):
pubmed: 34439128
Histopathology. 2010 Dec;57(6):862-76
pubmed: 21166700
Int J Oncol. 2014 May;44(5):1461-9
pubmed: 24589652
OMICS. 2017 Jul;21(7):390-403
pubmed: 28692419
Cancers (Basel). 2019 May 02;11(5):
pubmed: 31052560
Clin Cancer Res. 2018 May 15;24(10):2383-2394
pubmed: 29514845
Mol Cancer Res. 2021 Mar;19(3):414-428
pubmed: 33234576
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Oncogene. 2019 Dec;38(50):7447-7456
pubmed: 31427738
Cancers (Basel). 2021 May 21;13(11):
pubmed: 34063807
Cell Adh Migr. 2016 Nov;10(6):652-674
pubmed: 27533782
Cancer Res. 2002 Apr 15;62(8):2430-6
pubmed: 11956107
Front Pharmacol. 2019 May 21;10:542
pubmed: 31164821
Biochim Biophys Acta Rev Cancer. 2017 Apr;1867(2):151-161
pubmed: 28110020
Exp Clin Endocrinol Diabetes. 2020 Jun;128(6-07):462-468
pubmed: 31648351
BMC Cancer. 2019 Mar 29;19(1):289
pubmed: 30925904
Tumour Biol. 2015 Jan;36(1):409-19
pubmed: 25266803
Oncotarget. 2017 Jun 16;8(57):96945-96957
pubmed: 29228584
J Cancer. 2020 Feb 3;11(8):2252-2264
pubmed: 32127952
Nat Commun. 2017 Feb 09;8:14344
pubmed: 28181495
PLoS One. 2012;7(12):e51748
pubmed: 23284758
Oncogene. 2011 Jan 13;30(2):127-38
pubmed: 20890307
Transl Lung Cancer Res. 2017 Jun;6(3):343-349
pubmed: 28713679
Anticancer Res. 2014 Mar;34(3):1355-66
pubmed: 24596383
J Natl Cancer Inst. 2001 Dec 19;93(24):1886-8
pubmed: 11752014
J BUON. 2016 Sept-Oct;21(5):1203-1209
pubmed: 27837624
Cold Spring Harb Perspect Med. 2021 Sep 1;11(9):
pubmed: 34001525
Biosci Rep. 2020 Jul 31;40(7):
pubmed: 32519739
Front Cell Dev Biol. 2020 Apr 03;8:222
pubmed: 32309283
Acta Oncol. 2015 Jul;54(7):961-70
pubmed: 25984930
Mol Cancer. 2019 Mar 30;18(1):52
pubmed: 30925917
Cancers (Basel). 2020 Apr 13;12(4):
pubmed: 32295075
Respir Res. 2015 Jun 24;16:74
pubmed: 26104294
Genes (Basel). 2019 Jan 10;10(1):
pubmed: 30634629
Sci Rep. 2020 Mar 4;10(1):4025
pubmed: 32132574
Cancer Lett. 2020 Oct 1;489:41-49
pubmed: 32522693
Clin Exp Metastasis. 2007;24(8):673-83
pubmed: 18008173
Biomed Khim. 2019 Oct;65(6):457-467
pubmed: 31876516
Methods Mol Biol. 2018;1664:17-24
pubmed: 29019121
J Mol Cell Biol. 2019 Dec 19;11(12):1042-1055
pubmed: 30726932
Oncoimmunology. 2018 Oct 11;8(1):e1505174
pubmed: 30546943
Biochim Biophys Acta Proteins Proteom. 2019 Jan;1867(1):2-8
pubmed: 30392560
Front Endocrinol (Lausanne). 2022 Feb 11;13:800995
pubmed: 35222270
Cancer Immunol Immunother. 2022 Mar 7;:
pubmed: 35254477
Int J Clin Oncol. 2008 Feb;13(1):66-70
pubmed: 18307022
Cancer Biol Ther. 2006 May;5(5):518-22
pubmed: 16582595
J Proteome Res. 2015 Sep 4;14(9):3452-60
pubmed: 26155816
Cancer Genomics Proteomics. 2015 Jan-Feb;12(1):39-48
pubmed: 25560643
Anticancer Agents Med Chem. 2021 Dec 30;22(3):433-468
pubmed: 33687887
Thorac Cancer. 2020 Mar;11(3):704-712
pubmed: 31970893
Semin Oncol. 2014 Feb;41(1):5-16
pubmed: 24565577
Cancers (Basel). 2021 May 25;13(11):
pubmed: 34070230
Br J Cancer. 2017 Sep 26;117(7):994-1006
pubmed: 28772282
J Cell Biochem. 2019 Apr;120(4):6290-6298
pubmed: 30378158
Arch Pharm Res. 2019 Jan;42(1):40-47
pubmed: 30515725
Sci Adv. 2020 Jan 22;6(4):eaay9819
pubmed: 32010791
J Cell Sci. 2014 Jan 15;127(Pt 2):267-75
pubmed: 24421310
Cell. 2018 Oct 4;175(2):429-441.e16
pubmed: 30245008
Nature. 2009 Jan 1;457(7225):36-7
pubmed: 19122629
J Control Release. 2021 Mar 10;331:404-415
pubmed: 33485883
Cell Cycle. 2017 Apr 3;16(7):634-648
pubmed: 28166483
J Proteome Res. 2006 May;5(5):1143-54
pubmed: 16674103
Cell Death Dis. 2020 Oct 9;11(10):841
pubmed: 33037181
Exp Biol Med (Maywood). 2019 Jan;244(1):36-41
pubmed: 30776966
Anal Bioanal Chem. 2013 Jan;405(2-3):907-16
pubmed: 22842827
APMIS. 2004 Jul-Aug;112(7-8):431-40
pubmed: 15563307
Oncol Rep. 2018 Aug;40(2):669-681
pubmed: 29845263
Zhonghua Zhong Liu Za Zhi. 2015 Mar;37(3):165-9
pubmed: 25975782
Int J Mol Sci. 2021 Dec 31;23(1):
pubmed: 35008863
Signal Transduct Target Ther. 2021 Mar 10;6(1):117
pubmed: 33692331