Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
05 05 2022
05 05 2022
Historique:
received:
23
02
2021
accepted:
16
03
2022
entrez:
5
5
2022
pubmed:
6
5
2022
medline:
10
5
2022
Statut:
epublish
Résumé
SCCmec is a large mobile genetic element that includes the mecA gene and confers resistance to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus (MRSA). There is evidence that SCCmec disseminates among staphylococci, but the transfer mechanisms are unclear. Here, we show that two-component systems mediate the upregulation of natural competence genes in S. aureus under biofilm growth conditions, and this enhances the efficiency of natural transformation. We observe SCCmec transfer via natural transformation from MRSA, and from methicillin-resistant coagulase-negative staphylococci, to methicillin-sensitive S. aureus. The process requires the SCCmec recombinase genes ccrAB, and the stability of the transferred SCCmec varies depending on SCCmec types and recipients. Our results suggest that natural transformation plays a role in the transfer of SCCmec and possibly other mobile genetic elements in S. aureus biofilms.
Identifiants
pubmed: 35513365
doi: 10.1038/s41467-022-29877-2
pii: 10.1038/s41467-022-29877-2
pmc: PMC9072672
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2477Informations de copyright
© 2022. The Author(s).
Références
Drug Resist Updat. 2003 Feb;6(1):41-52
pubmed: 12654286
Antimicrob Agents Chemother. 2004 Jul;48(7):2637-51
pubmed: 15215121
Nat Rev Dis Primers. 2018 May 31;4:18033
pubmed: 29849094
Arch Virol. 2004 Sep;149(9):1689-703
pubmed: 15593413
Nat Rev Microbiol. 2009 Sep;7(9):629-41
pubmed: 19680247
Infect Immun. 2018 Sep 21;86(10):
pubmed: 30061377
J Biol Chem. 2013 Jan 4;288(1):132-40
pubmed: 23150671
J Vis Exp. 2017 Mar 10;(121):
pubmed: 28362383
Microbiology (Reading). 2019 Jul;165(7):779-791
pubmed: 31100054
PLoS Pathog. 2012;8(11):e1003003
pubmed: 23133387
Cell Mol Life Sci. 2010 Sep;67(18):3057-71
pubmed: 20668911
Cell Microbiol. 2012 Oct;14(10):1513-21
pubmed: 22747834
Int J Syst Evol Microbiol. 2020 Nov;70(11):5926-5936
pubmed: 33052802
Mol Microbiol. 2017 May;104(3):365-376
pubmed: 28142193
Microbiology (Reading). 2011 Aug;157(Pt 8):2206-2219
pubmed: 21565927
Antimicrob Agents Chemother. 2013 Apr;57(4):1968-70
pubmed: 23357771
Antimicrob Agents Chemother. 2004 Jun;48(6):2295-7
pubmed: 15155238
J Clin Invest. 2003 May;111(9):1265-73
pubmed: 12727914
Antimicrob Agents Chemother. 2013 Nov;57(11):5233-8
pubmed: 23939891
Environ Microbiol Rep. 2013 Feb;5(1):66-73
pubmed: 23757132
J Clin Microbiol. 1985 Dec;22(6):996-1006
pubmed: 3905855
Viruses. 2012 Dec;4(12):3316-35
pubmed: 23342361
Lancet. 2010 May 1;375(9725):1557-68
pubmed: 20206987
Mol Microbiol. 1999 Jan;31(1):271-80
pubmed: 9987128
Antimicrob Agents Chemother. 2010 Apr;54(4):1469-75
pubmed: 20086147
Antimicrob Agents Chemother. 1994 Nov;38(11):2590-8
pubmed: 7872753
Int J Med Microbiol. 2014 Jul;304(5-6):764-74
pubmed: 24951306
Mol Microbiol. 2016 May;100(4):675-85
pubmed: 26822382
Antimicrob Agents Chemother. 2000 Jun;44(6):1549-55
pubmed: 10817707
J Bacteriol. 2003 Sep;185(18):5465-72
pubmed: 12949098
Nat Commun. 2017 Oct 11;8(1):854
pubmed: 29021534
mBio. 2019 Aug 6;10(4):
pubmed: 31387900
Trends Microbiol. 2015 Jan;23(1):44-54
pubmed: 25455417
Curr Microbiol. 2018 Apr;75(4):499-504
pubmed: 29209822
J Microbiol Methods. 2011 Sep;86(3):379-82
pubmed: 21672561
Front Microbiol. 2018 Nov 13;9:2723
pubmed: 30483235
Trends Microbiol. 2017 Nov;25(11):893-905
pubmed: 28641931
FEMS Microbiol Rev. 2009 May;33(3):643-56
pubmed: 19228200
J Antimicrob Chemother. 2016 Mar;71(3):587-92
pubmed: 26661395
J Bacteriol. 1975 Sep;123(3):905-15
pubmed: 125746
BMC Microbiol. 2006 Oct 03;6:86
pubmed: 17014739
Nat Commun. 2018 Feb 6;9(1):523
pubmed: 29410457
J Bacteriol. 1970 Dec;104(3):1158-67
pubmed: 16559089
Emerg Infect Dis. 2018 Feb;24(2):242-248
pubmed: 29350135
J Med Microbiol. 2010 Oct;59(Pt 10):1135-1139
pubmed: 20616192
mBio. 2014 Oct 14;5(5):e01341-14
pubmed: 25316695
Mol Microbiol. 2014 Nov;94(3):557-79
pubmed: 25155269
Antimicrob Agents Chemother. 2019 Oct 22;63(11):
pubmed: 31501140
BMC Microbiol. 2011 Jan 18;11:13
pubmed: 21241511
Curr Top Microbiol Immunol. 2017;409:145-198
pubmed: 26728068
J Antimicrob Chemother. 2013 Apr;68(4):749-57
pubmed: 23249841
J Bacteriol. 2010 Jun;192(12):3204-12
pubmed: 20382769
Genes Cells. 2003 Aug;8(8):699-712
pubmed: 12875655
Microbiol Spectr. 2016 Apr;4(2):
pubmed: 27227309
J Bacteriol. 2008 Feb;190(4):1276-83
pubmed: 18083809
FEMS Microbiol Lett. 2005 Nov 1;252(1):89-96
pubmed: 16213676
Mol Microbiol. 2011 Aug;81(3):602-22
pubmed: 21696458
J Bacteriol. 2012 Jan;194(2):486-98
pubmed: 22056931
Antimicrob Agents Chemother. 2013 Jul;57(7):3037-45
pubmed: 23587945
Clin Microbiol Rev. 2018 Sep 12;31(4):
pubmed: 30209034
J Bacteriol. 2004 Feb;186(4):1175-81
pubmed: 14762013
Science. 1999 May 21;284(5418):1318-22
pubmed: 10334980
Antimicrob Agents Chemother. 2003 Dec;47(12):3926-34
pubmed: 14638503
mBio. 2019 Jun 25;10(3):
pubmed: 31239382
PLoS Pathog. 2010 May 13;6(5):e1000888
pubmed: 20485515
Int J Med Microbiol. 2010 Feb;300(2-3):98-103
pubmed: 19811948