Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.
Journal
Nature genetics
ISSN: 1546-1718
Titre abrégé: Nat Genet
Pays: United States
ID NLM: 9216904
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
24
05
2021
accepted:
15
03
2022
pubmed:
6
5
2022
medline:
20
5
2022
entrez:
5
5
2022
Statut:
ppublish
Résumé
Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we determined the phenotypes of more than 58,000 mutants under more than 121 different environmental growth conditions and chemical treatments. A total of 59% of genes are represented by at least one mutant that showed a phenotype, providing clues to the functions of thousands of genes. Mutant phenotypic profiles place uncharacterized genes into functional pathways such as DNA repair, photosynthesis, the CO
Identifiants
pubmed: 35513725
doi: 10.1038/s41588-022-01052-9
pii: 10.1038/s41588-022-01052-9
pmc: PMC9110296
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
705-714Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM119137
Pays : United States
Organisme : Howard Hughes Medical Institute
ID : 55108535
Pays : United States
Informations de copyright
© 2022. The Author(s).
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