A Novel Method for Total IgE Purification from Human Serum.
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 05 2022
15 05 2022
Historique:
received:
26
05
2021
accepted:
17
03
2022
pubmed:
7
5
2022
medline:
20
5
2022
entrez:
6
5
2022
Statut:
ppublish
Résumé
For Ab purification, high-affinity chromatography is commonly used. This technique results in high-purity Abs, but it requires highly specific knowledge and equipment. Commercial kits for purification of IgE are not available. Therefore, we established a (to our knowledge) novel method for the purification of total IgE from human serum. Sera from 19 allergic and nonallergic patients were included. After depletion of polyclonal IgG, total serum IgE was captured using anti-human IgE Abs coupled to beads, eluted from the beads, and incubated with protein G-coupled beads to increase the final purity. Purity analysis and Ab detection were performed by Western blot. Total serum IgE and purified IgE concentrations were analyzed using ELISA. To determine their functionality, primary human mast cells were sensitized with purified IgE and activated with anti-IgE or a relevant allergen. CD63
Identifiants
pubmed: 35523453
pii: jimmunol.2100497
doi: 10.4049/jimmunol.2100497
doi:
Substances chimiques
Allergens
0
Immunoglobulin G
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2436-2442Informations de copyright
Copyright © 2022 by The American Association of Immunologists, Inc.