Programming Receptor Clustering with DNA Probabilistic Circuits for Enhanced Natural Killer Cell Recognition.

Cell Recognition Cell Surface Engineering Cell-Based Immunotherapy DNA Probabilistic Circuits Receptor Clustering

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
11 07 2022
Historique:
received: 14 03 2022
pubmed: 7 5 2022
medline: 8 7 2022
entrez: 6 5 2022
Statut: ppublish

Résumé

Developing strategies to enhance the recognition ability of immune cells is important to the success of cell-based cancer immunotherapy. Herein, we report programming receptor clustering on membrane with DNA probabilistic circuits for enhanced immune cell recognition. By designing the circuit output to activate receptors for binding to adjacent receptors, we can engineer DNA probabilistic circuits for programmable regulation of receptor clustering. The generated receptor clusters show higher binding affinity to target cancer cells and improved membrane-anchoring stability compared with monomers. We demonstrate that programming receptor clustering could allow to modulate the recognition capability of natural killer cells and control natural killer cell-cancer cell interactions to promote efficient cancer cell killing. This work provides insights for precise control over cellular recognition and opens new opportunities for the development of cell-based immunotherapy.

Identifiants

pubmed: 35523723
doi: 10.1002/anie.202203800
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202203800

Informations de copyright

© 2022 Wiley-VCH GmbH.

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Auteurs

Mingshu Xiao (M)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China.

Wei Lai (W)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China.

Xiaowei Yao (X)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China.

Hao Pei (H)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China.

Chunhai Fan (C)

School of Chemistry and Chemical Engineering, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.

Li Li (L)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P. R. China.

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