Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin.
Journal
Nature neuroscience
ISSN: 1546-1726
Titre abrégé: Nat Neurosci
Pays: United States
ID NLM: 9809671
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
24
06
2021
accepted:
30
03
2022
pubmed:
7
5
2022
medline:
11
5
2022
entrez:
6
5
2022
Statut:
ppublish
Résumé
The mouse visual system serves as an accessible model to understand mammalian circuit wiring. Despite rich knowledge in retinal circuits, the long-range connectivity map from distinct retinal ganglion cell (RGC) types to diverse brain neuron types remains unknown. In this study, we developed an integrated approach, called Trans-Seq, to map RGCs to superior collicular (SC) circuits. Trans-Seq combines a fluorescent anterograde trans-synaptic tracer, consisting of codon-optimized wheat germ agglutinin fused to mCherry, with single-cell RNA sequencing. We used Trans-Seq to classify SC neuron types innervated by genetically defined RGC types and predicted a neuronal pair from αRGCs to Nephronectin-positive wide-field neurons (NPWFs). We validated this connection using genetic labeling, electrophysiology and retrograde tracing. We then used transcriptomic data from Trans-Seq to identify Nephronectin as a determinant for selective synaptic choice from αRGC to NPWFs via binding to Integrin α8β1. The Trans-Seq approach can be broadly applied for post-synaptic circuit discovery from genetically defined pre-synaptic neurons.
Identifiants
pubmed: 35524141
doi: 10.1038/s41593-022-01068-8
pii: 10.1038/s41593-022-01068-8
pmc: PMC9172271
mid: NIHMS1805325
doi:
Substances chimiques
Extracellular Matrix Proteins
0
nephronectin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
659-674Subventions
Organisme : NINDS NIH HHS
ID : R01 NS077986
Pays : United States
Organisme : NEI NIH HHS
ID : F30 EY033201
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS034661
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS123912
Pays : United States
Organisme : NIMH NIH HHS
ID : RF1 MH116989
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY030138
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS099099
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH081880
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH049428
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY002162
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.
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