Human derived tendon cells contribute to myotube formation in vitro.
Cell communication
Muscle regeneration
Myoblasts
Myogenesis
Myotube formation
Satellite cells
Skeletal muscle
Tendon
Tendon fibroblasts
Tenocytes
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
01 08 2022
01 08 2022
Historique:
received:
01
02
2022
revised:
15
04
2022
accepted:
18
04
2022
pubmed:
9
5
2022
medline:
9
6
2022
entrez:
8
5
2022
Statut:
ppublish
Résumé
Skeletal muscle possesses remarkable adaptability to mechanical loading and regenerative potential following muscle injury primarily due to satellite cell activity. Although the roles of several types of interstitial cells in skeletal muscle have been documented, the signaling interplay between the skeletal muscle and the adjacent tendon tissue has not been elucidated. Here, we tested whether human tendon derived cells (tenocytes) could induce human myogenic cells (myoblasts) proliferation and differentiation in vitro using co-culture experiments that allowed us to investigate the effect of tenocytes secretion upon myogenic progression. This was done in vitro by introducing insert wells with either myoblasts, tenocytes, or no cells (control) into a myoblast containing well (co-culture). Immunofluorescence analysis revealed a higher fusion index (≥5 nuclei within one Desmin
Identifiants
pubmed: 35526568
pii: S0014-4827(22)00157-4
doi: 10.1016/j.yexcr.2022.113164
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113164Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.