The splicing-regulatory lncRNA NTRAS sustains vascular integrity.
alternative splicing
long non-coding RNA
tight junctions
vascular integrity
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
07 06 2022
07 06 2022
Historique:
revised:
17
02
2022
received:
14
10
2021
accepted:
12
04
2022
pubmed:
10
5
2022
medline:
9
6
2022
entrez:
9
5
2022
Statut:
ppublish
Résumé
Vascular integrity is essential for organ homeostasis to prevent edema formation and infiltration of inflammatory cells. Long non-coding RNAs (lncRNAs) are important regulators of gene expression and often expressed in a cell type-specific manner. By screening for endothelial-enriched lncRNAs, we identified the undescribed lncRNA NTRAS to control endothelial cell functions. Silencing of NTRAS induces endothelial cell dysfunction in vitro and increases vascular permeability and lethality in mice. Biochemical analysis revealed that NTRAS, through its CA-dinucleotide repeat motif, sequesters the splicing regulator hnRNPL to control alternative splicing of tight junction protein 1 (TJP1; also named zona occludens 1, ZO-1) pre-mRNA. Deletion of the hnRNPL binding motif in mice (Ntras
Identifiants
pubmed: 35527520
doi: 10.15252/embr.202154157
pmc: PMC9171682
doi:
Substances chimiques
Protein Isoforms
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e54157Informations de copyright
© 2022 The Authors. Published under the terms of the CC BY 4.0 license.
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