Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-based Test on HEK-293T hACE2 Cells.
COVID-19
Immune response
Neutralizing antibody
Pseudo-type virus
SARS-CoV-2
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Apr 2022
05 Apr 2022
Historique:
received:
18
02
2022
revised:
11
11
2021
accepted:
25
02
2022
entrez:
9
5
2022
pubmed:
10
5
2022
medline:
10
5
2022
Statut:
epublish
Résumé
Neutralizing antibodies (NAbs) are of particular importance because they can prevent binding of the receptor binding domain (RBD) of the spike protein (S protein) to the angiotensin-converting enzyme 2 (ACE2) receptor present at the surface of human cells, preventing virus entry into the host cells. The gold standard method for detection of NAbs is the plaque reduction neutralization test (PRNT). Based on the measurement of cell lysis due to viral infection, this test is able to detect antibodies that prevent cell infection (Muruato
Identifiants
pubmed: 35530524
doi: 10.21769/BioProtoc.4377
pii: e4377
pmc: PMC9018432
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e4377Informations de copyright
Copyright © 2022 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsAll authors have none to declare.
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