To beat the heat - engineering of the most thermostable pyruvate decarboxylase to date.
Journal
RSC advances
ISSN: 2046-2069
Titre abrégé: RSC Adv
Pays: England
ID NLM: 101581657
Informations de publication
Date de publication:
18 Sep 2019
18 Sep 2019
Historique:
received:
11
08
2019
accepted:
03
09
2019
entrez:
9
5
2022
pubmed:
20
9
2019
medline:
20
9
2019
Statut:
epublish
Résumé
Pyruvate decarboxylase (PDC) is a key enzyme for the production of ethanol at high temperatures and for cell-free butanol synthesis. Thermostable, organic solvent stable PDC was evolved from bacterial PDCs. The new variant shows >1500-fold-improved half-life at 75 °C and >5000-fold-increased half-life in the presence of 9 vol% butanol at 50 °C.
Identifiants
pubmed: 35531508
doi: 10.1039/c9ra06251c
pii: c9ra06251c
pmc: PMC9071941
doi:
Types de publication
Journal Article
Langues
eng
Pagination
29743-29746Informations de copyright
This journal is © The Royal Society of Chemistry.
Déclaration de conflit d'intérêts
There are no conflicts to declare.
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