Panel sequencing links rare, likely damaging gene variants with distinct clinical phenotypes and outcomes in juvenile-onset SLE.

SLE childhood genetic jSLE juvenile-onset monogenic paediatric stratification

Journal

Rheumatology (Oxford, England)
ISSN: 1462-0332
Titre abrégé: Rheumatology (Oxford)
Pays: England
ID NLM: 100883501

Informations de publication

Date de publication:
23 02 2023
Historique:
received: 20 10 2021
revised: 22 04 2022
pubmed: 10 5 2022
medline: 3 3 2023
entrez: 9 5 2022
Statut: ppublish

Résumé

Juvenile-onset systemic lupus erythematosus (jSLE) affects 15-20% of lupus patients. Clinical heterogeneity between racial groups, age groups and individual patients suggests variable pathophysiology. This study aimed to identify highly penetrant damaging mutations in genes associated with SLE/SLE-like disease in a large national cohort (UK JSLE Cohort Study) and compare demographic, clinical and laboratory features in patient sub-cohorts with 'genetic' SLE vs remaining SLE patients. Based on a sequencing panel designed in 2018, target enrichment and next-generation sequencing were performed in 348 patients to identify damaging gene variants. Findings were integrated with demographic, clinical and treatment related datasets. Damaging gene variants were identified in ∼3.5% of jSLE patients. When compared with the remaining cohort, 'genetic' SLE affected younger children and more Black African/Caribbean patients. 'Genetic' SLE patients exhibited less organ involvement and damage, and neuropsychiatric involvement developed over time. Less aggressive first line treatment was chosen in 'genetic' SLE patients, but more second and third line agents were used. 'Genetic' SLE associated with anti-dsDNA antibody positivity at diagnosis and reduced ANA, anti-LA and anti-Sm antibody positivity at last visit. Approximately 3.5% of jSLE patients present damaging gene variants associated with younger age at onset, and distinct clinical features. As less commonly observed after treatment induction, in 'genetic' SLE, autoantibody positivity may be the result of tissue damage and explain reduced immune complex-mediated renal and haematological involvement. Routine sequencing could allow for patient stratification, risk assessment and target-directed treatment, thereby increasing efficacy and reducing toxicity.

Identifiants

pubmed: 35532072
pii: 6582551
doi: 10.1093/rheumatology/keac275
pmc: PMC9949710
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

SI210-SI225

Subventions

Organisme : Medical Research Council
ID : MR/R013926/1
Pays : United Kingdom

Informations de copyright

© The Author(s) 2022. Published by Oxford University Press on behalf of the British Society for Rheumatology.

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Auteurs

Amandine Charras (A)

Department of Women's & Children's Health, Institute of Life Course and Medical Sciences.

Sam Haldenby (S)

Centre for Genomic Research, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool.

Eve M D Smith (EMD)

Department of Women's & Children's Health, Institute of Life Course and Medical Sciences.
Department of Paediatric Rheumatology, Alder Hey Children's NHS Foundation Trust Hospital, Liverpool, UK.

Naomi Egbivwie (N)

Department of Women's & Children's Health, Institute of Life Course and Medical Sciences.
Department of Paediatric Rheumatology, Alder Hey Children's NHS Foundation Trust Hospital, Liverpool, UK.

Lisa Olohan (L)

Centre for Genomic Research, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool.

John G Kenny (JG)

Centre for Genomic Research, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool.
Teagasc Food Research Centre, Moorepark, Cork, Ireland.

Klaus Schwarz (K)

Institut for Transfusion Medicine, University Ulm, Ulm.
Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Service Baden-Württemberg-Hessen, Ulm, Germany.

Carla Roberts (C)

Department of Women's & Children's Health, Institute of Life Course and Medical Sciences.

Eslam Al-Abadi (E)

Department of Rheumatology, Birmingham Children's Hospital, Birmingham.

Kate Armon (K)

Department of Paediatric Rheumatology, Cambridge University Hospitals, Cambridge.

Kathryn Bailey (K)

Department of Paediatric Rheumatology, Oxford University Hospitals NHS Foundation Trust, Oxford.

Coziana Ciurtin (C)

Centre for Adolescent Rheumatology, University College London, London.

Janet Gardner-Medwin (J)

Department of Child Health, University of Glasgow, Glasgow.

Kirsty Haslam (K)

Department of Paediatrics, Bradford Royal Infirmary, Bradford.

Daniel P Hawley (DP)

Department of Paediatric Rheumatology, Sheffield Children's Hospital, Sheffield.

Alice Leahy (A)

Department of Paediatric Rheumatology, Southampton General Hospital, Southampton.

Valentina Leone (V)

Department of Paediatric Rheumatology, Leeds Children Hospital, Leeds.

Flora McErlane (F)

Paediatric Rheumatology, Great North Children's Hospital, Royal Victoria Infirmary, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne.

Gita Modgil (G)

Department of Paediatrics, Musgrove Park Hospital, Taunton.

Clarissa Pilkington (C)

Department of Paediatric Rheumatology, Great Ormond Street Hospital, London.

Athimalaipet V Ramanan (AV)

University Hospitals Bristol NHS Foundation Trust & Bristol Medical School, University of Bristol, Bristol.

Satyapal Rangaraj (S)

Department of Paediatric Rheumatology, Nottingham University Hospitals, Nottingham.

Phil Riley (P)

Department of Paediatric Rheumatology, Royal Manchester Children's Hospital, Manchester.

Arani Sridhar (A)

Department of Paediatrics, Leicester Royal Infirmary, Leicester, UK.

Michael W Beresford (MW)

Department of Women's & Children's Health, Institute of Life Course and Medical Sciences.
Centre for Genomic Research, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool.

Christian M Hedrich (CM)

Department of Women's & Children's Health, Institute of Life Course and Medical Sciences.
Centre for Genomic Research, Institute of Infection, Veterinary & Ecological Sciences, University of Liverpool.

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