Novel animal model of combined generalized and focal epilepsy.
animal model
combined generalized and focal epilepsy
oxidative stress
thioredoxin
Journal
Epilepsia
ISSN: 1528-1167
Titre abrégé: Epilepsia
Pays: United States
ID NLM: 2983306R
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
revised:
06
05
2022
received:
01
11
2021
accepted:
06
05
2022
pubmed:
10
5
2022
medline:
15
7
2022
entrez:
9
5
2022
Statut:
ppublish
Résumé
Thioredoxin, encoded by Txn1, is a critical antioxidant that protects against oxidative damage by regulating the dithiol/disulfide balance of interacting proteins. We recently discovered the Adem rat, an epileptic rat harboring the Txn1-F54L mutation, characterized by wild running and vacuolar degeneration in the midbrain. This study aimed to characterize the classification of epilepsy in Adem rats. We performed simultaneous video-electroencephalographic recordings, magnetic resonance imaging, neurotransmitter measurements using gas chromatography-mass spectrometry (GC-MS), and immunohistochemistry. Adem rats exhibited absence, tonic, and focal seizures. The type of epilepsy was classified as combined generalized and focal epilepsy. Neurotransmitters in the midbrain and cortex were measured at 3 weeks of age, when neuronal cell death occurs in the midbrain. The results of GC-MS ruled out the dominance of the excitatory system in the midbrain and cortex of Adem rats. Activation of astrocytes and microglia was more pronounced at 5 weeks of age, at which time epileptic seizures occurred frequently. The underlying pathology in Adem rats remains unknown. However, glial cell activation and inflammation may play a significant role in the occurrence of epilepsy.
Identifiants
pubmed: 35532890
doi: 10.1111/epi.17295
pmc: PMC9544055
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e80-e85Informations de copyright
© 2022 The Authors. Epilepsia published by Wiley Periodicals LLC on behalf of International League Against Epilepsy.
Références
Seizure. 2011 Jun;20(5):359-68
pubmed: 21292505
Neuropharmacology. 2020 Aug 1;172:107907
pubmed: 31837825
Sci Rep. 2016 Nov 09;6:36749
pubmed: 27827456
Prog Brain Res. 2016;226:195-207
pubmed: 27323944
Curr Opin Neurol. 2007 Apr;20(2):164-8
pubmed: 17351486
Seizure. 2018 Dec;63:17-25
pubmed: 30391662
Front Mol Neurosci. 2019 Apr 11;12:88
pubmed: 31031592
Nat Neurosci. 2015 Mar;18(3):339-43
pubmed: 25710835
Free Radic Biol Med. 2014 Jan;66:75-87
pubmed: 23899494
Neuron. 2014 Jan 22;81(2):229-48
pubmed: 24462092
Free Radic Biol Med. 2017 May;106:270-277
pubmed: 28232204
Epilepsia. 2022 Jul;63(7):e80-e85
pubmed: 35532890
Neurology. 2019 Jul 16;93(3):e215-e226
pubmed: 31227617
Nat Rev Neurol. 2011 Jan;7(1):31-40
pubmed: 21135885
Trends Neurosci. 2020 Mar;43(3):144-154
pubmed: 32044129
Bone Marrow Transplant. 2013 Mar;48(3):452-8
pubmed: 23208313
Nat Methods. 2018 Jan;15(1):53-56
pubmed: 29176591
Neuropsychiatr Dis Treat. 2014 Sep 09;10:1693-705
pubmed: 25228809