Temporal variation in bacterial community profile on patients' bedsheets in a primary healthcare unit.
Amplicon sequencing
Bacterial diversity
Patients’ bedsheets
Primary healthcare unit
Seasonal variation
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
10 May 2022
10 May 2022
Historique:
received:
27
01
2022
accepted:
13
04
2022
revised:
10
03
2022
entrez:
9
5
2022
pubmed:
10
5
2022
medline:
12
5
2022
Statut:
epublish
Résumé
Fabrics serve as fomites in spreading nosocomial infections. As a patient is in close contact with bedsheets, it is important to assess the seasonal variation in bacterial diversity on these in healthcare units. The study was conducted to characterise the bacterial diversity on patients' bedsheets across 7 months in a primary healthcare unit. Polyester-cotton blend fabric was stitched on bedsheets, and temporal dynamics of bacterial communities was assessed from May to November 2019. qPCR and amplicon sequencing of 16S rRNA gene was performed for profiling of bacterial community. Results revealed the dominance of Bacillota followed by Pseudomonadota, and Actinomycetota. A seasonal variation was observed in the bacterial load, with maximum values in June. This indicates the impact of environmental conditions on bacterial abundance and composition on fabrics in healthcare unit. The presence of priority pathogens on the patient bedsheets is a human health concern reiterating the need for season-specific laundering protocol.
Identifiants
pubmed: 35534776
doi: 10.1007/s00203-022-02921-x
pii: 10.1007/s00203-022-02921-x
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
308Subventions
Organisme : Indian Institute of Technology Delhi
ID : MI1798G
Organisme : Indian Institute of Technology Delhi
ID : MI1798G
Organisme : The Wellcome Trust DBT India Alliance
ID : IA/E/17/1/503700
Organisme : Department of Science and Technology, Ministry of Science and Technology
ID : INSPIRE fellowship IF160213
Organisme : University Grants Commission
ID : fellowship
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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