Exogenous expression of an allatotropin-related peptide receptor increased the membrane excitability in Aplysia neurons.
Aplysia
Electrophysiology
G-protein coupled receptors
Neuronal excitability
Neuropeptide
Plasmid microinjection
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
09 05 2022
09 05 2022
Historique:
received:
04
04
2022
accepted:
01
05
2022
entrez:
9
5
2022
pubmed:
10
5
2022
medline:
12
5
2022
Statut:
epublish
Résumé
Neuropeptides act mostly on a class of G-protein coupled receptors, and play a fundamental role in the functions of neural circuits underlying behaviors. However, physiological functions of some neuropeptide receptors are poorly understood. Here, we used the molluscan model system Aplysia and microinjected the exogenous neuropeptide receptor apATRPR (Aplysia allatotropin-related peptide receptor) with an expression vector (pNEX3) into Aplysia neurons that did not express the receptor endogenously. Physiological experiments demonstrated that apATRPR could mediate the excitability increase induced by its ligand, apATRP (Aplysia allatotropin-related peptide), in the Aplysia neurons that now express the receptor. This study provides a definitive evidence for a physiological function of a neuropeptide receptor in molluscan animals.
Identifiants
pubmed: 35534865
doi: 10.1186/s13041-022-00929-4
pii: 10.1186/s13041-022-00929-4
pmc: PMC9082908
doi:
Substances chimiques
Insect Hormones
0
Neuropeptides
0
Receptors, Neuropeptide
0
allatotropin
75831-28-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
42Informations de copyright
© 2022. The Author(s).
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