Towards spatially-organized organs-on-chip: Photopatterning cell-laden thiol-ene and methacryloyl hydrogels in a microfluidic device.
GelMA
GelNB
lymphocytes
methacrylate
organs-on-chip
photopolymerization
Journal
Organs-on-a-chip
ISSN: 2666-1020
Titre abrégé: Organs Chip
Pays: Netherlands
ID NLM: 9918383864506676
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
entrez:
10
5
2022
pubmed:
11
5
2022
medline:
11
5
2022
Statut:
ppublish
Résumé
Micropatterning techniques for 3D cell cultures enable the recreation of tissue-level structures, but the combination of patterned hydrogels with organs-on-chip to generate organized 3D cultures under microfluidic perfusion remains challenging. To address this technological gap, we developed a user-friendly in-situ micropatterning protocol that integrates photolithography of crosslinkable, cell-laden hydrogels with a simple microfluidic housing, and tested the impact of crosslinking chemistry on stability and spatial resolution. Working with gelatin functionalized with photo-crosslinkable moieties, we found that inclusion of cells at high densities (≥ 10
Identifiants
pubmed: 35535262
doi: 10.1016/j.ooc.2022.100018
pmc: PMC9078144
mid: NIHMS1801526
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM136615
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB029127
Pays : United States
Déclaration de conflit d'intérêts
Conflict of Interest Statement The authors have no conflicts to declare.
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