Detection of SARS-CoV-2 in different human biofluids using the loop-mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil.
RT-LAMP
coronavirus
molecular diagnosis
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
21
04
2022
received:
26
03
2022
accepted:
05
05
2022
pubmed:
11
5
2022
medline:
16
7
2022
entrez:
10
5
2022
Statut:
ppublish
Résumé
We adopted the reverse-transcriptase-loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) in patient samples. Two primer sets for genes N and Orf1ab were designed to detect SARS-CoV-2, and one primer set was designed to detect the human gene Actin. We collected prospective 138 nasopharyngeal swabs, 70 oropharyngeal swabs, 69 salivae, and 68 mouth saline wash samples from patients suspected to have severe acute respiratory syndrome (SARS) caused by SARS-CoV-2 to test the RT-LAMP in comparison with the gold standard technique reverse-transcription quantitative polymerase chain reaction (RT-qPCR). The accuracy of diagnosis using both primers, N5 and Orf9, was evaluated. Sensitivity and specificity for diagnosis were 96% (95% confidence interval [CI]: 87-99) and 85% (95% CI: 76-91) in 138 samples, respectively. Accurate diagnosis results were obtained only in nasopharyngeal swabs processed via extraction kit. Accurate and rapid diagnosis could aid coronavirus disease 2019 (COVID-19) pandemic management by identifying, isolating, and treating patients rapidly.
Identifiants
pubmed: 35535440
doi: 10.1002/jmv.27842
pmc: PMC9348339
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4170-4180Informations de copyright
© 2022 Wiley Periodicals LLC.
Références
J Clin Microbiol. 2006 Apr;44(4):1376-81
pubmed: 16597865
Arch Intern Med. 1994 Nov 28;154(22):2573-81
pubmed: 7979854
Appl Environ Microbiol. 2007 Sep;73(17):5660-2
pubmed: 17616628
Clin Microbiol Infect. 2020 Sep;26(9):1178-1182
pubmed: 32593741
Clin Microbiol Infect. 2020 Jun;26(6):773-779
pubmed: 32276116
Trends Analyt Chem. 2018 Jan;98:19-35
pubmed: 32287544
J Am Acad Dermatol. 2004 Dec;51(6):923-30
pubmed: 15583584
Nat Mater. 2021 May;20(5):593-605
pubmed: 33589798
Sci Rep. 2019 Mar 14;9(1):4494
pubmed: 30872672
J Med Microbiol. 2008 Apr;57(Pt 4):444-451
pubmed: 18349363
Int J Infect Dis. 2020 Aug;97:66-68
pubmed: 32492531
Nucleic Acids Res. 2000 Jun 15;28(12):E63
pubmed: 10871386
J Virol Methods. 2018 Mar;253:18-25
pubmed: 29253497
Mol Syst Biol. 2011 Oct 11;7:539
pubmed: 21988835
Clin Chem. 2020 Jul 1;66(7):975-977
pubmed: 32315390
Microb Biotechnol. 2020 Jul;13(4):950-961
pubmed: 32333644
J Orthop Res. 2017 Feb;35(2):361-365
pubmed: 27175946
Emerg Microbes Infect. 2020 Dec;9(1):998-1007
pubmed: 32306853
J Clin Diagn Res. 2016 Oct;10(10):YE01-YE06
pubmed: 27891446
Front Public Health. 2017 Nov 20;5:307
pubmed: 29209603
Indian J Microbiol. 2009 Dec;49(4):301-7
pubmed: 23100789
Biochim Biophys Acta. 1968 Jul 23;161(2):442-54
pubmed: 5667291
J Clin Microbiol. 2007 Feb;45(2):351-7
pubmed: 17135444
Virusdisease. 2020 Jun;31(2):97-105
pubmed: 32656306
J Virol Methods. 2014 Mar;197:14-8
pubmed: 24300833
J Virol Methods. 2006 Aug;135(2):272-5
pubmed: 16616961
Hum Pathol. 2005 Mar;36(3):303-9
pubmed: 15791576
J Med Virol. 2022 Sep;94(9):4170-4180
pubmed: 35535440
J Mol Diagn. 2020 Jun;22(6):729-735
pubmed: 32276051