Factor inhibiting HIF can catalyze two asparaginyl hydroxylations in VNVN motifs of ankyrin fold proteins.
JmjC demethylase
ankyrin
epigenetics
factor inhibiting HIF
hypoxia-inducible factor
iron and 2-oxoglutarate/alpha-ketoglutarate oxygenase
post-translational modification/hydroxylation
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
02
11
2021
revised:
28
03
2022
accepted:
15
04
2022
pubmed:
11
5
2022
medline:
30
6
2022
entrez:
10
5
2022
Statut:
ppublish
Résumé
The aspariginyl hydroxylase human factor inhibiting hypoxia-inducible factor (FIH) is an important regulator of the transcriptional activity of hypoxia-inducible factor. FIH also catalyzes the hydroxylation of asparaginyl and other residues in ankyrin repeat domain-containing proteins, including apoptosis stimulating of p53 protein (ASPP) family members. ASPP2 is reported to undergo a single FIH-catalyzed hydroxylation at Asn-986. We report biochemical and crystallographic evidence showing that FIH catalyzes the unprecedented post-translational hydroxylation of both asparaginyl residues in "VNVN" and related motifs of ankyrin repeat domains in ASPPs (i.e., ASPP1, ASPP2, and iASPP) and the related ASB11 and p18-INK4C proteins. Our biochemical results extend the substrate scope of FIH catalysis and may have implications for its biological roles, including in the hypoxic response and ASPP family function.
Identifiants
pubmed: 35537551
pii: S0021-9258(22)00460-4
doi: 10.1016/j.jbc.2022.102020
pmc: PMC9189129
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Apoptosis Regulatory Proteins
0
PPP1R13B protein, human
0
Repressor Proteins
0
TP53BP2 protein, human
0
Mixed Function Oxygenases
EC 1.-
HIF1AN protein, human
EC 1.14.11.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102020Subventions
Organisme : Medical Research Council
ID : MR/N021053/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 106244/Z/14/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
Références
J Biol Chem. 2003 Jan 17;278(3):1802-6
pubmed: 12446723
PLoS One. 2017 May 18;12(5):e0177656
pubmed: 28542244
Structure. 2008 Feb;16(2):259-68
pubmed: 18275817
Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14767-72
pubmed: 17003112
J Mol Biol. 2012 Nov 2;423(4):515-27
pubmed: 22917970
Science. 2001 Apr 20;292(5516):464-8
pubmed: 11292862
Ann N Y Acad Sci. 2009 Oct;1177:9-18
pubmed: 19845602
Genes Dev. 2002 Jun 15;16(12):1466-71
pubmed: 12080085
J Biol Chem. 2007 Aug 17;282(33):24027-38
pubmed: 17573339
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Biochem J. 2002 Nov 1;367(Pt 3):571-5
pubmed: 12215170
Science. 2001 Apr 20;292(5516):468-72
pubmed: 11292861
Mol Cell Biol. 2007 Sep;27(18):6407-19
pubmed: 17636018
Redox Biol. 2019 Sep;26:101265
pubmed: 31299612
J Biol Chem. 2011 Mar 4;286(9):7648-60
pubmed: 21177872
Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13331-6
pubmed: 25197067
Mol Biosyst. 2009 Jan;5(1):52-8
pubmed: 19081931
Mol Cell. 2008 May 23;30(4):393-402
pubmed: 18498744
FEBS J. 2011 Apr;278(7):1086-97
pubmed: 21251231
Annu Rev Biochem. 2018 Jun 20;87:585-620
pubmed: 29494239
Am J Physiol Cell Physiol. 2011 Sep;301(3):C550-2
pubmed: 21677261
J Biol Chem. 2021 Nov;297(5):101304
pubmed: 34655613
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4031-6
pubmed: 24550447
Nat Rev Mol Cell Biol. 2004 May;5(5):343-54
pubmed: 15122348
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):4019-24
pubmed: 24550462
Cell. 2001 Oct 5;107(1):43-54
pubmed: 11595184
J Mol Biol. 2009 Oct 2;392(4):994-1006
pubmed: 19646994
Mol Cell Proteomics. 2009 Mar;8(3):535-46
pubmed: 18936059
BMC Syst Biol. 2010 Oct 18;4:139
pubmed: 20955552
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
J Cell Sci. 2013 Jun 15;126(Pt 12):2629-40
pubmed: 23606740
Philos Trans R Soc Lond B Biol Sci. 2018 Jun 5;373(1748):
pubmed: 29685975
J Biol Chem. 2015 Aug 7;290(32):19726-42
pubmed: 26112411
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
J Biol Chem. 2008 Feb 15;283(7):3808-15
pubmed: 18063574
J Biol Chem. 2002 Jul 19;277(29):26351-5
pubmed: 12042299
Mol Pharmacol. 2014 May;85(5):651-7
pubmed: 24569087
Angew Chem Int Ed Engl. 2013 Feb 4;52(6):1700-4
pubmed: 23296631
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Cell. 2014 May 22;157(5):1130-45
pubmed: 24855949