Microfluidic device performing on flow study of serial cell-cell interactions of two cell populations.
Journal
RSC advances
ISSN: 2046-2069
Titre abrégé: RSC Adv
Pays: England
ID NLM: 101581657
Informations de publication
Date de publication:
09 Dec 2019
09 Dec 2019
Historique:
received:
14
11
2019
accepted:
05
12
2019
entrez:
11
5
2022
pubmed:
13
12
2019
medline:
13
12
2019
Statut:
epublish
Résumé
In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell-cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specific locations, then relied on hydrodynamic filtering principles, the flowing cells from the second cell population are placed in contact with the trapped ones, through a roll-over mechanism. The rolling cells interact with the serially trapped cells one after the other. The proposed microfluidic phenomenon was characterized with beads. We have shown the validity of our method by detecting the capacity of olfactory receptors to induce adhesion of cell doublets overexpressing these receptors. We report here the first controlled on-flow single cell resolution cell-cell interaction assay in a microfluidic device for future application in cell-cell interactions-based cell library screenings.
Identifiants
pubmed: 35540074
doi: 10.1039/c9ra09504g
pii: c9ra09504g
pmc: PMC9076435
doi:
Types de publication
Journal Article
Langues
eng
Pagination
41066-41073Informations de copyright
This journal is © The Royal Society of Chemistry.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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