A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
30
08
2021
accepted:
24
03
2022
pubmed:
12
5
2022
medline:
24
5
2022
entrez:
11
5
2022
Statut:
ppublish
Résumé
Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a flexible system that can address imaging applications with varied requirements in terms of resolution, sample size, tissue-clearing protocol, and transparent sample-holder material. Here, we present a 'hybrid' system that combines a unique non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet (OTLS) architecture for versatile multi-scale volumetric imaging. We demonstrate efficient screening and targeted sub-micrometer imaging of sparse axons within an intact, cleared mouse brain. The same system enables high-throughput automated imaging of multiple specimens, as spotlighted by a quantitative multi-scale analysis of brain metastases. Compared with existing academic and commercial light-sheet microscopy systems, our hybrid OTLS system provides a unique combination of versatility and performance necessary to satisfy the diverse requirements of a growing number of cleared-tissue imaging applications.
Identifiants
pubmed: 35545715
doi: 10.1038/s41592-022-01468-5
pii: 10.1038/s41592-022-01468-5
pmc: PMC9214839
mid: NIHMS1814331
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
613-619Subventions
Organisme : NCI NIH HHS
ID : R01 CA244170
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK107436
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NCI NIH HHS
ID : R00 CA240681
Pays : United States
Organisme : NCI NIH HHS
ID : K99 CA240681
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM079712
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS104949
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM104318
Pays : United States
Organisme : NIMH NIH HHS
ID : RF1 MH117820
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK092202
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB031002
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.
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