Rapid Capsular Antigen Immunoassay for Diagnosis of Inhalational Anthrax: Preclinical Studies and Evaluation in a Nonhuman Primate Model.
Bacillus anthracis
anthrax
capsule
diagnosis
immunoassay
immunoassays
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
28 06 2022
28 06 2022
Historique:
pubmed:
14
5
2022
medline:
1
7
2022
entrez:
13
5
2022
Statut:
ppublish
Résumé
Inhalational anthrax is a fatal infectious disease. Rapid and effective treatment is critically dependent on early and accurate diagnosis. Blood culture followed by identification and confirmation may take days to provide clinically relevant information. In contrast, immunoassay for a shed antigen, the capsular polypeptide gamma-d-polyglutamate (γDPGA), can provide rapid results at the point of care. In this study, a lateral flow immunoassay for γDPGA was evaluated in a robust nonhuman primate model of inhalational anthrax. The results showed that the time to a positive result with the rapid test using either serum or blood as a clinical specimen was similar to the time after infection when a blood culture became positive.
Identifiants
pubmed: 35546539
doi: 10.1128/mbio.00931-22
pmc: PMC9239138
doi:
Substances chimiques
Antigens, Bacterial
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0093122Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201000003C
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI059348
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI093365
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI061200
Pays : United States
Références
J Clin Invest. 2005 Mar;115(3):688-94
pubmed: 15696197
J Clin Microbiol. 2017 Oct;55(10):2964-2971
pubmed: 28747367
Infect Immun. 2007 Jan;75(1):152-63
pubmed: 17060470
MMWR Recomm Rep. 2015 Dec 04;64(4):1-22
pubmed: 26632963
Lett Appl Microbiol. 2019 Jun;68(6):480-484
pubmed: 30776143
J Biol Chem. 1958 Nov;233(5):1109-12
pubmed: 13598742
Emerg Infect Dis. 2017 Dec;23(13):
pubmed: 29155651
Clin Vaccine Immunol. 2012 Nov;19(11):1765-75
pubmed: 22956657
Anal Bioanal Chem. 2015 Apr;407(10):2847-58
pubmed: 25673244
Infect Immun. 2009 Aug;77(8):3432-41
pubmed: 19506008
J Exp Med. 1917 Oct 1;26(4):477-93
pubmed: 19868163
J Immunol Methods. 2014 Jun;408:78-88
pubmed: 24857756
PLoS One. 2007 May 23;2(5):e461
pubmed: 17520020
Emerg Infect Dis. 2002 Oct;8(10):1060-5
pubmed: 12396916
Nat Microbiol. 2019 Jan;4(1):46-54
pubmed: 30546093
Emerg Infect Dis. 1997 Apr-Jun;3(2):83-94
pubmed: 9204289
Emerg Infect Dis. 2002 Oct;8(10):1096-102
pubmed: 12396923
Lab Invest. 2003 Aug;83(8):1201-9
pubmed: 12920249
Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5042-7
pubmed: 15051894
Clin Vaccine Immunol. 2009 Mar;16(3):408-13
pubmed: 19129473
PLoS One. 2015 May 05;10(5):e0126304
pubmed: 25942409
EMBO J. 2005 Jan 12;24(1):221-7
pubmed: 15616593
J Bacteriol. 1958 Nov;76(5):499-503
pubmed: 13598708
Biochem J. 1946;40(2):297-309
pubmed: 20989452
Health Secur. 2015 Nov-Dec;13(6):365-77
pubmed: 26690378
FEMS Microbiol Lett. 2008 Aug;285(2):220-6
pubmed: 18549401