Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
27
04
2021
accepted:
11
04
2022
revised:
24
05
2022
pubmed:
14
5
2022
medline:
27
5
2022
entrez:
13
5
2022
Statut:
epublish
Résumé
Mucosal-associated invariant T (MAIT) cells are unconventional T lymphocytes with a semi-conserved TCRα, activated by the presentation of vitamin B metabolites by the MHC-I related protein, MR1, and with diverse innate and adaptive effector functions. The role of MAIT cells in acute intestinal infections, especially at the mucosal level, is not well known. Here, we analyzed the presence and phenotype of MAIT cells in duodenal biopsies and paired peripheral blood samples, in patients during and after culture-confirmed Vibrio cholerae O1 infection. Immunohistochemical staining of duodenal biopsies from cholera patients (n = 5, median age 32 years, range 26-44, 1 female) identified MAIT cells in the lamina propria of the crypts, but not the villi. By flow cytometry (n = 10, median age 31 years, range 23-36, 1 female), we showed that duodenal MAIT cells are more activated than peripheral MAIT cells (p < 0.01 across time points), although there were no significant differences between duodenal MAIT cells at day 2 and day 30. We found fecal markers of intestinal permeability and inflammation to be correlated with the loss of duodenal (but not peripheral) MAIT cells, and single-cell sequencing revealed differing T cell receptor usage between the duodenal and peripheral blood MAIT cells. In this preliminary report limited by a small sample size, we show that MAIT cells are present in the lamina propria of the duodenum during V. cholerae infection, and more activated than those in the blood. Future work into the trafficking and tissue-resident function of MAIT cells is warranted.
Identifiants
pubmed: 35551522
doi: 10.1371/journal.pntd.0010411
pii: PNTD-D-21-00600
pmc: PMC9129025
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0010411Subventions
Organisme : NIAID NIH HHS
ID : R01 AI130378
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI135115
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI058935
Pays : United States
Organisme : FIC NIH HHS
ID : D43 TW005572
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI106878
Pays : United States
Organisme : FIC NIH HHS
ID : K43 TW010362
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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